Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Systems analysis of uterine microenvironment 4, 6, 8, 10, 11 or 12 days after fertilization

ABSTRACT: Analysis of uterine microenvironment at gene expression level. The hypothesis tested in the present study was that Tregs orchestrated the immune reponse triggered in presence of embryo Total RNA obtained from uterine microenvironment tissues (4, 6, 8, 10, 11 or 12 days after fertilization) compared to control tissues (uterus from non pregnant mice).

ORGANISM(S): Mus musculus

SUBMITTER: Djamel NEHAR-BELAID

PROVIDER: E-GEOD-68433 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Regulatory T Cells Orchestrate Similar Immune Evasion of Fetuses and Tumors in Mice.

Nehar-Belaid Djamel D Courau Tristan T Dérian Nicolas N Florez Laura L Ruocco Maria Grazia MG Klatzmann David D

Journal of immunology (Baltimore, Md. : 1950) 20151207 2

Embryos and tumors are both masses of dividing cells expressing foreign Ags, but they are not rejected by the immune system. We hypothesized that similar tolerogenic mechanisms prevent their rejection. Global comparison of fetal and tumor microenvironments through transcriptomics in mice revealed strikingly similar and dramatic decreases in expression of numerous immune-related pathways, including Ag presentation and T cell signaling. Unsupervised analyses highlighted the parallel kinetics and s ...[more]

PMID: 26643476

Similar Datasets

Systems analysis of uterine microenvironment in Treg depleted pregnant mice at 12 days after fertilization

Project description:Analysis of uterine microenvironment at gene expression level. The hypothesis tested in the present study was that Tregs orchestrated the immune reponse triggered in presence of embryo. Total RNA obtained from uterine microenvironment tissues of Treg depleted pregnant mice (12 days after fertilization) compared to control tissues (uterus from PBS treated pregnant mice).

2015-12-10 | E-GEOD-68432 | biostudies-arrayexpress

Systems analysis of uterine microenvironment 4, 6, 8, 10, 11 or 12 days after fertilization

2015-12-10 | GSE68433 | GEO

Systems analysis of uterine microenvironment in Treg depleted pregnant mice at 12 days after fertilization

2015-12-10 | GSE68432 | GEO

The Deciphering of Mouse Uterine Microenvironment during Early Pregnancy [spatial transcriptomics]

Project description:The mouse uterine microenvironment at pregnancy D7.5 was investigated by Visium spatial transcriptome. Ten distinct uterine microenvironments were identified with diverse biological functions, different endocrine regulations and intensive interactions. The single cell RNA-seq of D7.5 mouse uterus has been intergrated with the spatial visium results and predicted the presence of mutiple types of cells in each microenvironment.

2022-07-30 | GSE181169 | GEO

The Deciphering of Mouse Uterine Microenvironment during Early Pregnancy [scRNA-seq]

2022-07-30 | GSE181168 | GEO

Systems analysis of B16-WT, B16-shTGFb and B16-shVEGF tumor microenvironment 4 or 14 days after tumor inoculation

Project description:Analysis of B16 tumor microenvironments at gene expression level. The hypothesis tested in the present study was that Tregs orchastrated the immune reponse triggered in presence of tumors Total RNA obtained from tumor microenvironment tissues (at Day 4 and Day 14 after tumor inoculation) compared to control tissues.

2016-09-01 | E-GEOD-73384 | biostudies-arrayexpress

Systems analysis of B16 tumor microenvironment 4 or 14 days after tumor inoculation

Project description:Analysis of B16 tumor microenvironment at gene expression level. The hypothesis tested in the present study was that Tregs orchastrated the immune reponse triggered in presence of tumors

2015-12-10 | GSE68434 | GEO

Resident effector memory Tregs of the uterus regulate trophic functions during mouse early pregnancy

Project description:Regulatory T cells (Tregs) contribute to successful pregnancy by promoting maternal-fetal tolerance. We identified a novel population of CD25-Foxp3+ resident uterine Tregs in non-pregnant mouse uterus. They have phenotypic and migration properties of tissue-resident effector-memory T cells (Trm), thus identifying regulatory Trm (regTrm). Lack of CD25 expression in regTrm is caused by the IL-2-deprived uterine microenvironment. We performed a complete transcriptome analysis by RNA-seq to determined whether uterine regTrm have a unique molecular signature, as has been described for other tissue Tregs. For this, we purified CD25- regTrm from non-pregnant and pregnant uterus at day 6 to 8 of pregnancy, and we compared them to CD4+Foxp3- (CD4conv) T cells from non-pregnant uterus and to CD25+ Tregs from paraaortic uterine draining lymph nodes. We used criteria of (i) a two-fold or greater change in expression and (ii) a p-value <0.05 (cut-off at 5% FDR) to determine differentially expressed genes (DEGs). DEGs were annotated by their functional relevance in pregnancy using ingenuity pathway analysis (IPA) and using the PubMed Query: (Gene main name OR Gene Synonymous 1 OR Gene Synonymous 2 OR …) AND "pregnancy" OR “regulatory T cells” OR "uterus". We found that uterine regTrm have a unique transcriptional profile, impregnated with a uterine signature comprising up-regulation of genes related to the endometrium and to uterine receptivity. Pregnancy triggers regTrm expression of genes involved in trophic functions such as uterine development, extracellular matrix remodeling, hypoxia and vasculogenesis.

2019-05-01 | GSE109895 | GEO

Uterine glands impact uterine receptivity, luminal fluid homeostasis and blastocyst implantation

Project description:Uterine glands are essential for pregnancy in mice and likely humans, because they secrete or transport bioactive substances that regulate uterine receptivity for blastocyst implantation. In mice, the uterus becomes receptive to blastocyst implantation on day 4, but is refractory by day 5. Here, blastocysts could be recovered from progesterone-induced uterine gland (PUGKO) but not wildtype (WT) mice on day 5 post-mating. Anti-adhesive Muc1 protein and microvilli were present on the luminal epithelium of PUGKO but not WT uteri. A number of known uterine receptivity genes and gland-specific genes were altered in the PUGKO uterus. Next, the uterus and uterine luminal fluid (ULF) were obtained from WT and PUGKO mice on day 3, 4 and 5. Transcriptome analysis revealed that 580 genes were decreased in the PUGKO uterus, however ULF secrotome analysis revealed that many proteins and several amino acids were increased in the PUGKO ULF. Of note, many proteins encoded by many gland-specific genes were not identified in the ULF of WT mice. These results support the ideas that uterine glands secrete factors that regulate ULF homeostasis and interact with other cell types in the uterus to influence uterine receptivity and blastocyst implantation for the establishment of pregnancy.

2016-11-22 | GSE90101 | GEO

Chicken uterine fluid protein description and quantification

Project description:Gallus gallus avian eggshell is a biomineral composed of 95% of calcium carbonate on calcitic form and of 3.5% of an extracellular organic matrix (proteins, polysaccharides and proteoglycans). The calcification process occurred in the lumen of the uterus into the acellular uterine fluid, and is divided in three stages (initial, growth and terminal), where the concentration and the nature of proteins varies depending on stages. We have used mass spectrometry methodologies to identify and quantify proteins secreted into the uterine fluid proteins during the three stages of biomineralization.

2014-08-18 | PXD000992 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data