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Identification of a hepatocyte stem cell and its niche in liver homeostasis

ABSTRACT: Summary: RNAseq analysis of Axin2+ hepatocyte stem cells versus Axin2- mature hepatocytes shows that Axin2+ cells have a distinct gene expression profile compared to Axin2- hepatocytes. Methods: Hepatocytes were isolated from 8 week old Axin2rtTA;TetO-H2B-GFP mice (n = 3) given doxycycline in drinking water for 7 days. After removal of non-parenchymal cells, the hepatocytes were sorted into GFP+ versus GFP- populations by FACS. mRNA was isolated and RNAseq profiles were generated using Illumina HiSeq 2000. The reads were mapped to the mouse genome (mm10) using TopHat (v2.0.11) and differential gene expression analysis was performed using CuffDiff (v.2.2.2). Results: We mapped about 60 million sequence reads per sample to the mouse genome (build mm10) and identified more than 30,000 transcripts in the Axin2+ and Axin2- cell populations. Differential gene expression analysis using CuffDiff identified 96 genes with signficantly different expression levels between the two populations. Several of these were confirmed by RNA in situ and qRT-PCR analysis. mRNA profiles of Axin2+ and Axin2- cells from uninjured adult mouse liver were analysed by deep sequencing, in triplicate, using Illumina HiSeq 2000.

ORGANISM(S): Mus musculus

SUBMITTER: Bruce Wang

PROVIDER: E-GEOD-68806 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of a hepatocyte stem cell and its niche in liver homeostasis

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2015-08-07 | GSE68806 | GEO

Expression data from mouse calvarial suture mesenchymal cells

Project description:Axin2-expressing calvarial suture stem cells can contribute to calvarial development, homeostatic maintenance, repair, and regeneration. We used microarray to examine the gene expression profiles of Axin2-expressing suture stem cells and Axin2-negative cells in suture mesenchyme. Three of Axin2+/GFP+ and three of Axin2-/GFP- cell samples were collected from mice carrying Axin2rtTA and TREH2BGFP transgenes. Each samples were isolated from 6-8 Axin2rtTA; TRE-H2BGFP mice and sorted by the GFP intensity.

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Transcriptome profiling of GATA4 depleted hepatocytes

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Transcriptome profiling of GATA4,6 double depleted hepatocytes

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2013-11-07 | E-GEOD-51478 | biostudies-arrayexpress

Project description:ATACseq of AXIN2-/+ hepatocytes and AXIN2+ intestinal stem cells (ISCs) and LGR4/5KO hepatocytes

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miR-125b: A Rheostat of Growth versus Stress-Induced MAPKs That Governs Stemness and OncomiR Addiction in Skin

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2023-03-11 | PXD027035 | Pride

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