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Exosomal natural antisense transcripts released from colorectal cancer SW480 cells into culture supernatants

ABSTRACT: In the present study, natural antisense transcripts (NATs), transcribed from reverse strand deoxynucleic acids (DNAs) of genes, into exosomes released from colorectal cancer SW480 cells were searched using an sense/antisense-custom microarray. SW480 cells were cultured with serum-free RPMI1640 for 48 h. Then, culture media were collected, centrifugated, and filtrated using 0.22-um filters. Exosomes from culture media were collected by ultracentrifugation.

ORGANISM(S): Homo sapiens

SUBMITTER: Mitsuru Chiba

PROVIDER: E-GEOD-68979 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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