Exosomal natural antisense transcripts released from colorectal cancer SW480 cells into culture supernatants
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ABSTRACT: In the present study, natural antisense transcripts (NATs), transcribed from reverse strand deoxynucleic acids (DNAs) of genes, into exosomes released from colorectal cancer SW480 cells were searched using an sense/antisense-custom microarray. SW480 cells were cultured with serum-free RPMI1640 for 48 h. Then, culture media were collected, centrifugated, and filtrated using 0.22-um filters. Exosomes from culture media were collected by ultracentrifugation.
ORGANISM(S): Homo sapiens
SUBMITTER: Mitsuru Chiba
PROVIDER: E-GEOD-68979 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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