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Gene expression data from mouse tracheal mesenchymal cells before and 48hrs after SO2 injury

ABSTRACT: The conducting airway epithelium of the rodent and human lung is underlaid by mesenchymal cells that include vasculature, smooth muscle, fibroblasts and cartilage. The goal of this project is to identify cellular and molecular changes in the mesenchyme after injury to the epithelium by exposure to SO2 and which may participate in repair of the epithelium We used Affymetrix microarray analysis to compare transcripts in tracheal mesenchyme before and after SO2 injury. Mice tracheal epithelium and mesenchyme were separate for RNA extraction before and 48hrs after SO2 injury. Sample from 4 mice were pooled for each biological experiment. The experiments were repeated three times for triplicate samples.

ORGANISM(S): Mus musculus

SUBMITTER: Xia Gao

PROVIDER: E-GEOD-69057 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression data from mouse tracheal epithelial cells isolated before and 48hrs after SO2 injury

Project description:The conducting airway epithelium of the rodent and human lung is made up of about equal proportions of ciliated and secretory cells. In addition, in regions where the epithelium is pseudostratfied, ~30% of the epithelium consists of undifferentiated basal cells (BCs). Evidence suggests that these BCs are multipotent stem cells that can self renew over the long term and give rise to both ciliated and secretory lineages. The goal of this project is to identify cellular and molecular mechanisms by which the basal cells normally maintain the epithelium and repair it after injury. We used Affymetrix microarray analysis to compare transcripts in tracheal epithelium before and after SO2 injury. Mice tracheal epithelium and mesenchyme were separate for RNA extraction before and 48hrs after SO2 injury. Cells from tracheas of 4 male C57Bl/6 mice were pooled for each biological experiment. The experiments were repeated three times for triplicate samples.

2016-07-03 | E-GEOD-69056 | biostudies-arrayexpress

Gene expression data from mouse tracheal mesenchymal cells before and 48hrs after SO2 injury

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2016-01-01 | GSE69057 | GEO

Gene expression data from mouse tracheal cells before and 48hrs after SO2 injury

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-07-03 | E-GEOD-69058 | biostudies-arrayexpress

Gene expression data from mouse tracheal epithelial cells isolated before and 48hrs after SO2 injury

2016-01-01 | GSE69056 | GEO

Airway-Associated Macrophages in Homeostasis and Repair

Project description:We isolated CD45+ cells after injury (Uninjured, Day1, Day4 and Day7) and CD45+F4/80+ cells from tracheal mesenchyme and dissociated lung, as well as CD45+ cells from peeled tracheal epithelium.

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Effects of SO2 on yeast metabolism and changes in the transcriptional profiles

Project description:Here we report the massively parallel cDNA sequencing (RNA-seq) analysis performed using high throughput sequencing of a vineyard yeast strain collected from the vineyards of the Piave AO (Appellation of origin) (R008 strains) region in North East of Italy and 3 commercial yeast strains (EC1118, VL3 and AWRI796). Yeast cells were collected at the same step of the fermentation curve: at the beginning of the process, when the CO2 produced by the cells was 6 g/l (middle exponential growth phase). The fermentations have been performed for each strain with no SO2 added and with SO2 added at a final concentration of 25 mg/l. Three biological replicates of the fermentations were performed for each strain and samples for RNA-seq were gathered at the beginning of the process. The aim of this experiment was the comparison of the transcriptomes of the four yeast strains to identify the genes characterizing sulphites response. Eight samples were analyzed: R008 strain at middle exponential growth phase (6 g/l CO2 produced) with no SO2 added (previously submitted with ID GSM1092510, experiment GSE44845), R008 strain at middle exponential growth phase (6 g/l CO2 produced) with 25mg/l of SO2 added, EC1118 strain at middle exponential growth phase (6 g/l CO2 produced) with no SO2 added (previously submitted with ID GSM1092506, experiment GSE44845), EC1118 strain at middle exponential growth phase (6 g/l CO2 produced) with 25mg/l of SO2 added, VL3 strain at middle exponential growth phase (6 g/l CO2 produced) with no SO2 added, VL3 strain at middle exponential growth phase (6 g/l CO2 produced) with 25mg/l of SO2 added, AWRI796 strain at middle exponential growth phase (6 g/l CO2 produced) with no SO2 added, AWRI796 strain at middle exponential growth phase (6 g/l CO2 produced) with 25mg/l of SO2 added.

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Gene expression data from mouse tracheal cells before and 48hrs after SO2 injury

Project description:Gene expression data from mouse tracheal cells before and 48hrs after SO2 injury

| PRJNA284410 | ENA

Cartilage rings contribute to the proper embryonic tracheal epithelial differentiation, metabolism and expression of inflammatory genes

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Transcription profiling of rat tracheal epithelial RTEC11 cells

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Transcription profiling of chicken tracheal cells grown in vitro and infected with influenza virus compared to trachea from in vivo infected animals

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