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Transcription profiling of rat tracheal epithelial RTEC11 cells

ABSTRACT: We performed global scale microarray analysis to identify detailed mechanisms by which nonpermissive temperature induces cell growth arrest and differentiation in tracheal epithelial RTEC11 cells harboring temperature-sensitive simian virus 40 large T-antigen by using an Affymetrix GeneChip system. Tracheal epithelial RTEC11 cells used in this study were derived from transgenic rats harboring a temperature-sensitive simian virus 40 large T-antigen. Although the cells grew continuously at the permissive temperature, the nonpermissive temperature led to cell growth arrest and differentiation. Experiment Overall Design: A rat conditionally immortalized tracheal epithelial cell line, RTEC11, derived from transgenic rats harboring a temperature-sensitive simian virus 40 large T-antigen was used. RTEC11 cells were cultured at permissive (33Â°C) or nonpermissive (39Â°C) temperature for 3 days.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Yoshiaki Tabuchi

PROVIDER: E-GEOD-8444 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genes in nonpermissive temperature-induced cell growth arrest and differentiation of tracheal epithelial RTEC11 cells

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