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Wheat: Control vs. Pathogen-infected

ABSTRACT: Differentially expressed kinase genes in Rhizoctonia cerealis resistant wheat lines CI12633/Shanhongmai compared with the susceptible wheat line Wenmai 6 via Agilent Wheat Gene Expression Microarray assay. Goal was to identify the kinase genes whose expression was higher in CI12633/Shanhongmai compared with the susceptible wheat line Wenmai 6 CI12633/Shanhongmai vs. Wenmai 6

ORGANISM(S): Triticum aestivum

SUBMITTER: Xiuliang Zhu

PROVIDER: E-GEOD-69245 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The wheat AGC kinase TaAGC1 is a positive contributor to host resistance to the necrotrophic pathogen Rhizoctonia cerealis.

Zhu Xiuliang X Yang Kun K Wei Xuening X Zhang Qiaofeng Q Rong Wei W Du Lipu L Ye Xingguo X Qi Lin L Zhang Zengyan Z

Journal of experimental botany 20150727 21

Considerable progress has been made in understanding the roles of AGC kinases in mammalian systems. However, very little is known about the roles of AGC kinases in wheat (Triticum aestivum). The necrotrophic fungus Rhizoctonia cerealis is the major pathogen of the destructive disease sharp eyespot of wheat. In this study, the wheat AGC kinase gene TaAGC1, responding to R. cerealis infection, was isolated, and its properties and role in wheat defence were characterized. R. cerealis-resistant whea ...[more]

PMID: 26220083

Similar Datasets

Wheat: Control vs. Pathogen-infected

Project description:Differentially expressed kinase genes in Rhizoctonia cerealis resistant wheat lines CI12633/Shanhongmai compared with the susceptible wheat line Wenmai 6 via Agilent Wheat Gene Expression Microarray assay. Goal was to identify the kinase genes whose expression was higher in CI12633/Shanhongmai compared with the susceptible wheat line Wenmai 6

2015-08-06 | GSE69245 | GEO

Global gene expression analysis of FHB disease spreading mechanism in wheat

Project description:The Fusarium graminearum is responsible for Fusarium head blight (FHB) disease in wheat which makes unsuitable for end-use quality of wheat. For that reason several studies have been aiming to develop FHB resistant cultivars. In order to clarify possible wheat response mechanism, global gene expression analysis in response to the pathogen was undertaken. The study was conducted using three genotype of wheat, Japanese cultivar Nobeokabouzu-komugi (Highly resistant), cv. Sumai 3 (Resistant) and cv. Gamenya (Susceptible) which florets were inoculated Fusarium graminearum M-bM-^@M-^\H-3M-bM-^@M-^] strain during the flowering stage. For microarray analysis used samples of 3 and 7 days after inoculation (DAI) and Agilent wheat custom array 4x38k. Over 1400 genes were differentially up-regulated in Nobeokabouzu-komugi at 3 DAI followed by Sumai 3 and minimum expression in Gamenya, whereas at 7 DAI in Sumai 3 observed high expression and minimum in Gamenya. Further narrowing down by removing commonly expressed genes in three genotypes and grouping according to the gene function, identified differentially high expression of genes involved in detoxification process such as multidrug resistant rpoteins, UDP-glycosyltransferase and ABC transporters in Nobeokabouzu-komugi at 3 DAI. However in Sumai 3 found many localized defense-related genes such as peroxidase, proteases and genes involved in plant cell wall defense at 7 DAI. Following in silico analysis, revealed those genes were located in different chromosomes. This finding showed the differentiation of molecular response mechanism among the FHB resistant cultivars in response to the pathogen. F. graminearum inoculated (3 and 7 days after inoculation) and water inoculated Mock samples were used for cRNA synthesis and further hybridization with 3 biological replications. Total 12 samples per genotype

2015-01-30 | E-GEOD-59721 | biostudies-arrayexpress

Gene expression profiles during long-term low-temperature treatment and cold acclimation in synthetic wheat hexaploids

Project description:We conducted microarray analysis to study comprehensive changes of gene expression profile under long-term low-temperature (LT) treatment and to identify other LT-responsive genes related with cold acclimation in seedling leaves and crown tissues (shoots containing apical meristems) of a synthetic hexaploid wheat line. The microarray analysis revealed marked up-regulation of a number of Cor/Lea genes and fructan biosynthesis-related genes under the long-term LT treatment. For validation of the microarray data, we selected four synthetic wheat lines, which contained the A and B genomes from a tetraploid wheat cultivar Langdon and the diverse D genomes originating from the different Ae. tauschii accessions, with distinct levels of freezing tolerance after cold acclimation. Quantitative RT-PCR analyses showed that the transcription accumulated levels of the Cor/Lea, CBF, and fructan biosynthesis-related genes were higher in more freezing-tolerant lines than those in the sensitive lines. The fructan biosynthesis pathway would be associated with cold acclimation to develop wheat freezing tolerance and related with diversity of the freezing tolerance level in addition to the CBF-mediated Cor/Lea expression pathway. Expression patterns were compared between a synthetic wheat line which treated 24M-bM-^DM-^C and 4M-bM-^DM-^C. Total RNA samples were respectively isolated from leaves and crown tissues of the synthetic line grown at normal temperature for 3 weeks and then at 4M-BM-0C for 12 and 6 weeks. Two independent experiments were conducted in each exprement.

2014-10-10 | E-GEOD-61621 | biostudies-arrayexpress

Microarray analysis of leaf rust-induced bread wheat genes

Project description:One week old bread wheat plantlets were artifically infected with Puccinia triticinae (the causal organism of wheat leaf rust) and samples were collected after one week from infection. Samples were collected after one week from infection, non infected as well. Two loacl varities were used MISR 1 and GEMMZA 7. Four chamber slide was used and single dye (Cy3) was detedcted. Non infected samples from both varities were hybridized in two chamber seperately and the other two chamber were assigned for the two infected samples.

2014-07-20 | E-GEOD-55297 | biostudies-arrayexpress

Comparative proteomics analysis of virulent and less virulent strains of Rhizoctonia solani

Project description:Comparative proteomics analysis of a virulent and a less virulent strains of Rhizoctonia solani was carried out using LC-MS/MS (Label free quantitation).

2022-05-19 | PXD023430 | Pride

Expression data in wheat (T. aestivum L.) near isogenic lines in response to powdery mildew infection

Project description:We used two wheat genotypes, the susceptible wheat cultivar ‘8866 ’(S) and its near isogenic line with single powdery mildew resistance gene ‘pm30’ (R), to investigate gene expression changes in response to powdery mildew infection by using Wheat Genome Array wheat young leveas of near isogenic lines before or 12 hours after powdery mildew infection were selected for RNA extraction and hybridization on Affymetrix microarrays.The leaf samples were harvested from three independent biological replicates, and the leaves without inoculation were regarded as control.

2011-02-15 | E-GEOD-27320 | biostudies-arrayexpress

Expression data from wheat cultivars

Project description:To identify genes involved in susceptibility, genechip hybridization experiments were performed in order to examine genes differentially expressed upon inoculation of resistant and susceptible wheat cultivars with powdery mildew. Some genes were identified which were just expressed in the susceptible host both after mock-inoculation and pathogen infection. Also, a total of 2693 transcripts were differentially expressed (fold change≥2) in Yumai 13 in response to powdery mildew as compared to itself, comprising 1464 and 1229 up- and down-regulated genes respectively. Seven-day-old wheat seedlings of susceptible cultivar Yumai 13, two resistant cultivars HY and CYC were inoculated with powdery mildew and harvested at 0, 24, 48 hpi for RNA extraction and hybridization on Affymetrix microarrays. We sought to screen some genes which have very high expression in Yumai 13, but not in CYC and HY by pairwise comparation.

2014-01-30 | E-GEOD-43930 | biostudies-arrayexpress

Transcription analysis by RNAseq of adult wheat after inoculation with Puccinia graminis f. sp. tritici

Project description:The RNA sequencing analysis was undertaken to investigate the transcriptomic changes in adult wheat inoculated with Puccinia graminis f. sp. tritici the causal agent of stem rust disease. The project firstly aims to compare gene expression in one susceptible wheat line with two wheat lines exhibiting adult plant resistance to the stem rust. Secondly, the project aims to examine the temporal changes in gene expression in wheat after inoculation. Wheat plants was grown until maturity under greenhouse conditions. Plants were inoculated with Puccinia graminis f. sp. tritici and the flag leaf sheath sampled for RNA sequencing. The project aims to give essential insight into the adult plant resistance response in wheat to Puccinia graminis f. sp. tritici inoculation.

2022-10-30 | E-MTAB-9681 | biostudies-arrayexpress

Expression data from wheat following Hessian fly larval attack.

Project description:The Hessian fly (HF, Mayetiola destructor) is a biotrophic insect that interacts with wheat on a typical gene-for-gene basis. Identification of the genes which are differentially expressed during wheat-HF interactions may provide critical information to better understand the plant resistance mechanisms. Microarray analyses of transcripts, including those encoding various lipases, lipid transfer proteins, enzymes involved in oxylipin synthesis, and enzymes involved in wax and cutin synthesis, revealed that the abundance of many of these transcripts increased rapidly in resistant plants after HF attack, but did not change in susceptible plants. We conducted the genome-wide transcriptional analysis of gene expression to identify the genes from wheat plants which were differentially express during compatible and incompatible wheat-Hessian fly interactions at 6, 12, and 24 hrs. Two wheat lines M-bM-^@M-^\MollyM-bM-^@M-^] and M-bM-^@M-^\NewtonM-bM-^@M-^] were used in the experiment. Molly contains R gene H13 and have incompatible interaction with Hessian fly larvae whereas Newton is susceptible and has compatible interaction with Hessian fly larvae. Total RNA was extracted from larval feeding site which is 1.5-2 cm of the second leaf sheath and hybridized on Affymetrix microarrays. Tissues were collected at three time points (6, 12, and 24 hr) after the Hessian fly egg hatching. The data obtained from each treatment were compared with the uninfested control within each wheat line under identical conditions.

2012-10-01 | E-GEOD-34445 | biostudies-arrayexpress

Trichoderma reesei transcript levels during mycoparasitism

Project description:To investigate the potential role in mycoparasitism, microarrays were used to examine T. reesei transcript levels when confronted with a potential prey (the plant pathogen Rhizoctonia solani) before contact, during first physical contact and during overgrowth of the host. Two biological pools by condition against a common reference control each sample hybridized in dye switch. On the two biological replicates we apply on the pretreated results the linear modeling approach implemented by lmFit and the empirical Bayes statistics implemented by eBayes from the limma R package (Smyth 2004).

2011-07-01 | E-GEOD-23410 | biostudies-arrayexpress

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