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The ISW1 and CHD1 ATP-dependent chromatin remodelers compete to set nucleosome spacing in vivo

ABSTRACT: We addressed the roles of three nucleosome spacing enzymes (ISW1, ISW2 and CHD1) in specifying chromatin organization in S. cerevisiae. Comparison of budding yeast nucleosome positions obtained using micrococcal nuclease digestion followed by paired-end sequencing (MNase-seq). We sequenced nucleosomes from isogenic strains with the isw1Î, isw2Î or chd1Î mutations in all combinations. We measured gene activity by ChIP-seq for the Rpb3 subunit of RNA polymerase II for some of these mutants.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Razvan Chereji

PROVIDER: E-GEOD-69400 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The ISW1 and CHD1 ATP-dependent chromatin remodelers compete to set nucleosome spacing in vivo.

Ocampo Josefina J Chereji Răzvan V RV Eriksson Peter R PR Clark David J DJ

Nucleic acids research 20160209 10

Adenosine triphosphate-dependent chromatin remodeling machines play a central role in gene regulation by manipulating chromatin structure. Most genes have a nucleosome-depleted region at the promoter and an array of regularly spaced nucleosomes phased relative to the transcription start site. In vitro, the three known yeast nucleosome spacing enzymes (CHD1, ISW1 and ISW2) form arrays with different spacing. We used genome-wide nucleosome sequencing to determine whether these enzymes space nucleo ...[more]

PMID: 26861626

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The ISW1 and CHD1 ATP-dependent chromatin remodelers compete to set nucleosome spacing in vivo

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