Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide identification of FHY3 binding sites in Arabidopsis floral organ (ChIP-Seq)


ABSTRACT: To identify FHY3 associated genes in floral organ. We performed ChIP-seq using 35S:3FLAG-FHY3-3HA fhy3-4 tansgenic plants. A total of 21, 24 and 37 million reads were obtained from two replicates and input library, respectively, which were uniquely mapped to the Arabidopsis genome by Bowtie2 software resulting in 1885 FHY3 binding peaks. 84% of FHY3 binding sites (1580 loci) were subsequently assigned to genic regions and grouped into 2192 genes in flower. Inflorescence of 35S:3FLAG-FHY3-3HA fhy3-4 containing stage 8 and younger flowers were harvested for ChIP-seq analysis with anti-FLAG antibodies. No antibody served as negative control. The chromatin DNA from two distinct biological replicates and an input DNA sample were subjected to ultra-high-throughput Solexa (Illumina) sequencing.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Xing Fu 

PROVIDER: E-GEOD-69422 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

FAR-RED ELONGATED HYPOCOTYL3 activates SEPALLATA2 but inhibits CLAVATA3 to regulate meristem determinacy and maintenance in Arabidopsis.

Li Dongming D   Fu Xing X   Guo Lin L   Huang Zhigang Z   Li Yongpeng Y   Liu Yang Y   He Zishan Z   Cao Xiuwei X   Ma Xiaohan X   Zhao Meicheng M   Zhu Guohui G   Xiao Langtao L   Wang Haiyang H   Chen Xuemei X   Liu Renyi R   Liu Xigang X  

Proceedings of the National Academy of Sciences of the United States of America 20160728 33


Plant meristems are responsible for the generation of all plant tissues and organs. Here we show that the transcription factor (TF) FAR-RED ELONGATED HYPOCOTYL3 (FHY3) plays an important role in both floral meristem (FM) determinacy and shoot apical meristem maintenance in Arabidopsis, in addition to its well-known multifaceted roles in plant growth and development during the vegetative stage. Through genetic analyses, we show that WUSCHEL (WUS) and CLAVATA3 (CLV3), two central players in the es  ...[more]

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