Unknown,Transcriptomics,Genomics,Proteomics

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EHMT1 and EHMT2 inhibition induce fetal hemoglobin expression [ChIP-seq]


ABSTRACT: Using UNC0638 and genetic assays to inhibit EHMT1/2 and derepress fetal hemoglobin in adult hematopoietic cells. ChIP-seq for 2 histone modifications and total H3 (for quantitative normalization) in biological triplicates of cord blood, adult bone marrow, and UNC0638-treated adult bone marrow.

ORGANISM(S): Homo sapiens

SUBMITTER: Peter van Galen 

PROVIDER: E-GEOD-69516 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications


Fetal hemoglobin (HbF, α2γ2) induction is a well-validated strategy for sickle cell disease (SCD) treatment. Using a small-molecule screen, we found that UNC0638, a selective inhibitor of EHMT1 and EHMT2 histone methyltransferases, induces γ-globin expression. EHMT1/2 catalyze mono- and dimethylation of lysine 9 on histone 3 (H3K9), raising the possibility that H3K9Me2, a repressive chromatin mark, plays a role in silencing γ-globin expression. In primary human adult erythroid cells, UNC0638 and  ...[more]

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