Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression profiling of thymocytes from conditional knock-out mice (FoxN1-Cre mediated KO of Gpr177)


ABSTRACT: Comparative gene expression profiling of thymocytes at the DP, CD4 SP and CD8 SP stage derived from FoxN1-Gpr177 mice (FoxN1-Cre mediated deletion of (Exon3 of) Gpr177/Wtls) or C57Bl/6N mice as comparison. Objective was to test the influence of TEC-secreted Wnt ligands on the transcriptome of thymocytes at the respective developmental stages. Total RNA extracted from FACS-sorted primary mouse thymocytes. CD4/8 double positive (DP) thymocytes, CD4 single positive (CD4 SP) thymocytes and CD8 single positive (CD8 SP) thymocytes were FACS-sorted from conditional knock-out mice (FoxN1-Gpr177) and C57Bl/6N mice as comparison.

ORGANISM(S): Mus musculus

SUBMITTER: Fabian Brunk 

PROVIDER: E-GEOD-69766 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Thymic Epithelial Cells Are a Nonredundant Source of Wnt Ligands for Thymus Development.

Brunk Fabian F   Augustin Iris I   Meister Michael M   Boutros Michael M   Kyewski Bruno B  

Journal of immunology (Baltimore, Md. : 1950) 20151028 11


Wnt signaling has been implicated in T cell development. However, it remained unclear which cell type is the major source of Wnt ligands and to what extent thymic epithelial cell (TEC) development is dependent on Wnt signaling. In this study, we analyzed the role of Wnt ligands provided by TECs for the development of T cells and TECs without manipulating the intracellular Wnt signaling machinery in either cell type. To this end, we used conditional knockout mice (FoxN1-Gpr177) in which TECs are  ...[more]

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