Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

IHH mRNA response to genetically-reconstructed Zaire Ebola viruses [mRNA]


ABSTRACT: The purpose of this experiment was to obtain samples for mRNA analysis in IHH cells infected with Zaire Ebola virus and mutants: Zaire Ebola virus: This wild-type Ebola virus - strain Mayinga - was isolated from a fatal human case in Zaire (now known as the Democratic Republic of Congo) in 1976 Zaire Ebola virus, VP35 R312A possesses a R312A mutation in the VP35 protein. Zaire Ebola virus, delta sGP. Lacks the ability to produce non-structural protein, the secreted glycoprotein (sGP). Zaire Ebola virus, delta mucin. Lacks the mucin-like domain (MLD), which contains both N-linked and O-linked glycosylation sites, for the glycoproteins. Overview of Experiment: Cells: Immortalized Human Hepatocytes (IHH); seed 60,000 cells per well in a 24-well plate. Infected with a multiplicity of infection (MOI) of 0.5. After infection, 3x wash with PBS and replace with 5% FCS DMEM without NaPyr or NEAA. Time matched mocks done in triplicate from same cell stock as rest of samples. Time Points = 0, 6, 12, 24, 48, and 72 hrs post infection in triplicate.

ORGANISM(S): Homo sapiens

SUBMITTER: Yoshihiro Kawaoka 

PROVIDER: E-GEOD-69942 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2015-06-18 | E-GEOD-69943 | biostudies-arrayexpress
2015-06-18 | GSE69943 | GEO
2015-06-18 | GSE69942 | GEO
2016-05-19 | E-GEOD-80832 | biostudies-arrayexpress
2016-05-07 | GSE80059 | GEO
2016-05-07 | GSE80058 | GEO
2021-01-30 | E-MTAB-10008 | biostudies-arrayexpress
2015-02-04 | E-GEOD-65573 | biostudies-arrayexpress
2022-08-02 | GSE210189 | GEO
2016-09-30 | E-GEOD-80832 | ExpressionAtlas