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Human liver Huh cell transcriptome (mRNA) response to a Wild-type ΔVP30 Ebola virus, Δmucin, and ssGP mutants

ABSTRACT: The purpose is to obtain samples for mRNA analysis in human liver Huh cells using the following viruses obtained by reverse genetics: Zaire Ebola virus wild-type (WT) in the ΔVP30 background (wild), ΔsGP virus (eliminates expression of soluble glycoprotein [GP]) (ssGP), and Δmucin virus (encodes a GP lacking the mucin domain) (mucin).

ORGANISM(S): Homo sapiens

PROVIDER: GSE80058 | GEO | 2016/05/07

SECONDARY ACCESSION(S): PRJNA317724

REPOSITORIES: GEO

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Human liver Huh cell transcriptome (miRNA) response to a Wild-type ΔVP30 Ebola virus, Δmucin, and ssGP mutants

Project description:The purpose is to obtain samples for microRNA analysis in human liver Huh cells using the following viruses obtained by reverse genetics: Zaire Ebola virus wild-type (WT) in the ΔVP30 background (wild), ΔsGP virus (eliminates expression of soluble glycoprotein [GP]) (ssGP), and Δmucin virus (encodes a GP lacking the mucin domain) (mucin).

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Project description:The purpose is to obtain samples for mRNA analysis in human U937 cells infected with Zaire Ebola virus wild-type in the deltaVP30 background and delta-mucin virus. Human U937 cells (monocyte-like) expressing the Ebola VP30 protein were infected with Zaire Ebola virus wild-type (wild) in the delta-VP30 background and delta-mucin virus (encodes a GP lacking the mucin domain) (mucin). Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 0, 8, 18, and 30 h post-infection.

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IHH microRNA response to genetically-reconstructed Zaire Ebola viruses [miRNA]

Project description:The purpose of this experiment was to obtain samples for mRNA analysis in IHH cells infected with Zaire Ebola virus and mutants: Zaire Ebola virus: This wild-type Ebola virus - strain Mayinga - was isolated from a fatal human case in Zaire (now known as the Democratic Republic of Congo) in 1976 Zaire Ebola virus, VP35 R312A possesses a R312A mutation in the VP35 protein. Zaire Ebola virus, delta sGP. Lacks the ability to produce non-structural protein, the secreted glycoprotein (sGP). Zaire Ebola virus, delta mucin. Lacks the mucin-like domain (MLD), which contains both N-linked and O-linked glycosylation sites, for the glycoproteins. Overview of Experiment: Cells: Immortalized Human Hepatocytes (IHH); seed 60,000 cells per well in a 24-well plate. Infected with a multiplicity of infection (MOI) of 0.5. After infection, 3x wash with PBS and replace with 5% FCS DMEM without NaPyr or NEAA. Time matched mocks done in triplicate from same cell stock as rest of samples. Time Points = 0, 6, 12, 24, 48, and 72 hrs post infection in triplicate.

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