Unknown,Transcriptomics,Genomics,Proteomics

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MLX transcriptional regulation in muscle cells

ABSTRACT: This study set out to identify MLX transcriptional targets in muscle cells. C2C12 Myoblasts were virally transduced to increase MLX activity, by overexpression of the wild-type protein; and to decrease MLX activity by overexpression of a dominant negative MLX protein and by shRNA induced knockdown of MLX. Transcripts that were significantly and consistently regulated by the different modes of MLX modulation were identified. The largest proportion of these were genes encoding secreted proteins including growth factors, cytokines and extracellular proteins. We therefore conclude that MLX can regulate myokine transcripts. mRNA profiles from C2C12 muscle cells with increased and decreased MLX activity were examined.

ORGANISM(S): Mus musculus

SUBMITTER: David Finkelstein

PROVIDER: E-GEOD-70028 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The glucose-sensing transcription factor MLX promotes myogenesis via myokine signaling.

Hunt Liam C LC Xu Beisi B Finkelstein David D Fan Yiping Y Carroll Patrick A PA Cheng Pei-Feng PF Eisenman Robert N RN Demontis Fabio F

Genes & development 20151119 23

Metabolic stress and changes in nutrient levels modulate many aspects of skeletal muscle function during aging and disease. Growth factors and cytokines secreted by skeletal muscle, known as myokines, are important signaling factors, but it is largely unknown whether they modulate muscle growth and differentiation in response to nutrients. Here, we found that changes in glucose levels increase the activity of the glucose-responsive transcription factor MLX (Max-like protein X), which promotes an ...[more]

PMID: 26584623

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Dataset Information

MLX transcriptional regulation in muscle cells

Publications

The glucose-sensing transcription factor MLX promotes myogenesis via myokine signaling.

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