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Oxygen-dependent transcriptome profiling in R.sphaeroides 2.4.1 in the dark

ABSTRACT: Wild type (2.4.1) strain, dark conditions, controlled oxtgen concentration in the growth medium (achieved by constant sparging with a gas mixture containing 1% CO2, variable - indicated for every sample - concentration O2 and balance N2). Keywords: dose response Cells were harvested at OD 0.16-0.18 after rapid addition of rifampicin, spun down and frozen for subsequent RNA isolation. RNA was isolated using bead beater and RNeasy kit. Traces of DNA were removed by DNAse I treatment until level non-detectable by 33-36 cycles of real-time PCR. Further sample preparaton -according Affymetrix protocols. Data processing: MAS 5.0 values are deposited here (for compatability with other submitted samples), values used in the publications are obtained with RMA and may be recreated using the supplied CEL files.

ORGANISM(S): Rhodobacter sphaeroides

SUBMITTER: Mark Gomelsky

PROVIDER: E-GEOD-7004 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Novel heme-based oxygen sensor with a revealing evolutionary history.

Moskvin Oleg V OV Kaplan Samuel S Gilles-Gonzalez Marie-Alda MA Gomelsky Mark M

The Journal of biological chemistry 20070727 39

To monitor fluctuations in oxygen concentration, cells use sensory proteins often containing heme cofactors. Here, we identify a new class of heme-binding oxygen sensors, reveal their unusual phylogenetic origin, and propose a sensing mode of a member of this class. We show that heme is bound noncovalently to the central region of AppA, an oxygen and light sensor from Rhodobacter sphaeroides. The addition of oxygen to ferrous AppA discoordinated the heme, and subsequent oxygen removal fully rest ...[more]

PMID: 17660296

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