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M1A marks translation initiation sites in human and mouse messenger RNA [Sequencing]

ABSTRACT: We developed a novel approach, m1A-seq, for high-resolution mapping of the transcriptome-wide m1A landscape, based on antibody-mediated capture followed by massively parallel sequencing Identification of m1A modified sequences in human, mouse and yeast cell lines

ORGANISM(S): Homo sapiens

SUBMITTER: Sharon Moshitch-Moshkovitz

PROVIDER: E-GEOD-70485 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The dynamic N(1)-methyladenosine methylome in eukaryotic messenger RNA.

Dominissini Dan D Nachtergaele Sigrid S Moshitch-Moshkovitz Sharon S Peer Eyal E Kol Nitzan N Ben-Haim Moshe Shay MS Dai Qing Q Di Segni Ayelet A Salmon-Divon Mali M Clark Wesley C WC Zheng Guanqun G Pan Tao T Solomon Oz O Eyal Eran E Hershkovitz Vera V Han Dali D Doré Louis C LC Amariglio Ninette N Rechavi Gideon G He Chuan C

Nature 20160210 7591

Gene expression can be regulated post-transcriptionally through dynamic and reversible RNA modifications. A recent noteworthy example is N(6)-methyladenosine (m(6)A), which affects messenger RNA (mRNA) localization, stability, translation and splicing. Here we report on a new mRNA modification, N(1)-methyladenosine (m(1)A), that occurs on thousands of different gene transcripts in eukaryotic cells, from yeast to mammals, at an estimated average transcript stoichiometry of 20% in humans. Employin ...[more]

PMID: 26863196

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