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MMSET alters EZH2 Binding in Myeloma Cells

ABSTRACT: We investigated EZH2 binding in the presence and absence of MMSET protein. MMSET overexpression in t(4;14)+ myeloma leads to global loss of H3K27 methylation and redistribution of EZH2 binding throughout the genome ChIP-seq for EZH2 in two cell types

ORGANISM(S): Homo sapiens

SUBMITTER: Relja Popovic

PROVIDER: E-GEOD-57632 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Histone methyltransferase MMSET/NSD2 alters EZH2 binding and reprograms the myeloma epigenome through global and focal changes in H3K36 and H3K27 methylation.

Popovic Relja R Martinez-Garcia Eva E Giannopoulou Eugenia G EG Zhang Quanwei Q Zhang Qingyang Q Ezponda Teresa T Shah Mrinal Y MY Zheng Yupeng Y Will Christine M CM Small Eliza C EC Hua Youjia Y Bulic Marinka M Jiang Yanwen Y Carrara Matteo M Calogero Raffaele A RA Kath William L WL Kelleher Neil L NL Wang Ji-Ping JP Elemento Olivier O Licht Jonathan D JD

PLoS genetics 20140904 9

Overexpression of the histone methyltransferase MMSET in t(4;14)+ multiple myeloma patients is believed to be the driving factor in the pathogenesis of this subtype of myeloma. MMSET catalyzes dimethylation of lysine 36 on histone H3 (H3K36me2), and its overexpression causes a global increase in H3K36me2, redistributing this mark in a broad, elevated level across the genome. Here, we demonstrate that an increased level of MMSET also induces a global reduction of lysine 27 trimethylation on histo ...[more]

PMID: 25188243

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