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Infection exposure promotes ETV6-RUNX1 precursor B cell leukemia revealing secondary genomic alterations in histone modification genes

ABSTRACT: We used microarrays to investigate gene expression changes in ETV6/RUNX1 proB and preB cells Bone marrow proB and preB cells of four ETV6/RUNX1 mice compared with bone marrow proB and preB cells of three WT mice and three Pax5+/- mice.

ORGANISM(S): Mus musculus

SUBMITTER: Isidro Sánchez García

PROVIDER: E-GEOD-70492 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Infection exposure promotes ETV6-RUNX1 precursor B cell leukemia revealing secondary genomic alterations in histone modification genes

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Project description:ETV6-RUNX1 is a first-hit mutation in childhood B cell precursor acute lymphoblastic leukaemia. ETV6-RUNX1 is a fusion protein which inherits the DNA-binding runt domain from RUNX1. Here we performed chromatin precipitation for native RUNX1 and ETV6-RUNX1 using RUNX1 antibodies and specifically for the ETV6-RUNX1 fusion using a V5-tag pull down.

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