Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: sun baofa
PROVIDER: E-GEOD-71096 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Xiao Wen W Adhikari Samir S Dahal Ujwal U Chen Yu-Sheng YS Hao Ya-Juan YJ Sun Bao-Fa BF Sun Hui-Ying HY Li Ang A Ping Xiao-Li XL Lai Wei-Yi WY Wang Xing X Ma Hai-Li HL Huang Chun-Min CM Yang Ying Y Huang Niu N Jiang Gui-Bin GB Wang Hai-Lin HL Zhou Qi Q Wang Xiu-Jie XJ Zhao Yong-Liang YL Yang Yun-Gui YG
Molecular cell 20160211 4
The regulatory role of N(6)-methyladenosine (m(6)A) and its nuclear binding protein YTHDC1 in pre-mRNA splicing remains an enigma. Here we show that YTHDC1 promotes exon inclusion in targeted mRNAs through recruiting pre-mRNA splicing factor SRSF3 (SRp20) while blocking SRSF10 (SRp38) mRNA binding. Transcriptome assay with PAR-CLIP-seq analysis revealed that YTHDC1-regulated exon-inclusion patterns were similar to those of SRSF3 but opposite of SRSF10. In vitro pull-down assay illustrated a comp ...[more]