Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression data of sorted GFP+ and tdTomato+ prostate tumor cells from Pten/Smad4/mTmG mice

ABSTRACT: Single cells from Ptenpc-/-Smad4pc-/-mTmG+ prostate tumors were isolated into single cells which were FACS-sorted for GFP+ and Tomato+ cells and RNA was purified with TRIzol (Life Technologies). RNA expression profiling was performed using the Mouse Genome 430 2.0 Array (Affymetrix) to generate a Ptenpc-/-Smad4pc-/- tumor/Stroma dataset. Two mice (9074, 9076) at 28 weeks old were used to isolate anterior prostate (AP) and dorsolateral prostate (DLP). From each prostate lobe, three populations were extracted for RNA: unsorted, GFP+, Tomato+. Therefore, there are total 12 samples.

ORGANISM(S): Mus musculus

SUBMITTER: Alan Wang

PROVIDER: E-GEOD-71319 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Targeting YAP-Dependent MDSC Infiltration Impairs Tumor Progression.

Wang Guocan G Lu Xin X Dey Prasenjit P Deng Pingna P Wu Chia Chin CC Jiang Shan S Fang Zhuangna Z Zhao Kun K Konaparthi Ramakrishna R Hua Sujun S Zhang Jianhua J Li-Ning-Tapia Elsa M EM Kapoor Avnish A Wu Chang-Jiun CJ Patel Neelay Bhaskar NB Guo Zhenglin Z Ramamoorthy Vandhana V Tieu Trang N TN Heffernan Tim T Zhao Di D Shang Xiaoying X Khadka Sunada S Hou Pingping P Hu Baoli B Jin Eun-Jung EJ Yao Wantong W Pan Xiaolu X Ding Zhihu Z Shi Yanxia Y Li Liren L Chang Qing Q Troncoso Patricia P Logothetis Christopher J CJ McArthur Mark J MJ Chin Lynda L Wang Y Alan YA DePinho Ronald A RA

Cancer discovery 20151223 1

<h4>Unlabelled</h4>The signaling mechanisms between prostate cancer cells and infiltrating immune cells may illuminate novel therapeutic approaches. Here, utilizing a prostate adenocarcinoma model driven by loss of Pten and Smad4, we identify polymorphonuclear myeloid-derived suppressor cells (MDSC) as the major infiltrating immune cell type, and depletion of MDSCs blocks progression. Employing a novel dual reporter prostate cancer model, epithelial and stromal transcriptomic profiling identifie ...[more]

PMID: 26701088

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Project description:Background Human monocytotropic ehrlichiosis is an emerging life-threatening zoonosis caused by obligately intracellular bacterium, Ehrlichia chaffeensis. E. chaffeensis is transmitted by the lone star tick, Amblyomma americanum, and replicates in mononuclear phagocytes in mammalian hosts. Differences in the E. chaffeensis transcriptome in mammalian and arthropod hosts are unknown. Thus, we determined host-specific E. chaffeensis gene expression in human monocyte (THP-1) and in Amblyomma and Ixodes tick cell lines (AAE2 and ISE6) using a whole genome microarray. Methodology/Principal Findings The majority (~80%) of E. chaffeensis genes were expressed during infection in human and tick cells. There were few differences observed in E. chaffeensis gene expression between the vector Amblyomma and non-vector Ixodes tick cells, but extensive host-specific and differential gene expression profiles were detected between human and tick cells, including higher transcriptional activity in tick cells and identification of gene subsets that were differentially expressed in the two hosts. Differentially and host-specifically expressed ehrlichial genes encoded major immunoreactive tandem repeat proteins (TRP), the outer membrane protein (OMP-1) family, and hypothetical proteins that were 30–80 amino acids in length. Consistent with previous observations, high expression of p28 and OMP-1B genes was detected in human and tick cells, respectively. Notably, E. chaffeensis genes encoding TRP32 and TRP47 were highly upregulated in the human monocytes and expressed as proteins; however, although TRP transcripts were expressed in tick cells, the proteins were not detected in whole cell lysates demonstrating that TRP expression was post transcriptionally regulated. Conclusions/Significance Ehrlichia gene expression is highly active in tick cells, and differential gene expression among a wide variety of host-pathogen associated genes occurs. Furthermore, we demonstrate that genes associated with host-pathogen interactions are differentially expressed and regulated by post transcriptional mechanisms. A microarray (4-plex) study using E. chaffeensis cultivated in each cell line (THP-1, AAE2 and ISE6), three biological replicates/cell line. For each cell line, RNA was also extracted from uninfected cells (negative controls) and was processed similar to the infected cells; these samples were used for background subtraction during data analysis.

Dataset Information

Expression data of sorted GFP+ and tdTomato+ prostate tumor cells from Pten/Smad4/mTmG mice

Publications

Targeting YAP-Dependent MDSC Infiltration Impairs Tumor Progression.

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