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NIK expression stimulated G6PD phosphorylation

ABSTRACT: Our findings establish NIK as a pivotal regulator of T cell metabolism in anti-tumor immunity and highlight a posttranslational mechanism of metabolic regulation involving the G6PD-NADPH redox system. CoIP assays revealed a strong physical interaction between NIK and G6PD in both T cells and transiently transfected 293 cells, suggesting G6PD to be a direct target of NIK. Using a phosphoprotein gel analysis approach, we demonstrated that NIK expression stimulated G6PD phosphorylation. To further study the mechanism, we performed mass spectrometry identify phosphorylation sites of G6PD stimulated by NIK

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Meidi Gu

LAB HEAD: David H. Hawke, Ph.D.

PROVIDER: PXD020943 | Pride | 2020-10-05

REPOSITORIES: Pride

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			Action	DRS
	050419ncs-GU-4-KA-phospho.mzML	Mzml
	050419ncs-GU-4-KA-phospho.mzid.gz	Mzid
	050419ncs-GU-4-KA-phospho.raw	Raw
	050519ncs-GU-3-KA-control.mzML	Mzml
	050519ncs-GU-3-KA-control.mzid.gz	Mzid

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