Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of mouse adultbrain hemispheres

ABSTRACT: Inbred congenic strain B6.C6.132.54/Vad was created using C57BL/6ByJ background and BALB/cJ donor strains. Flanking background markers at chr. 6: 75.9 Mb (rs4226008, NCBI Mouse Build 36 / dbSNP Build 126) and 122.3 Mb (rs3023093), and limiting donor markers at 81.9 Mb (rs4226024) and at 91.8 Mb (rs3712161) defined the introgressed region. We concluded the segment size must be between 9.9 Mb and 46.4 Mb. In a Quantitative Trait Gene identification study we compared brain (without cerebellum) gene expression between progenitors and congenics. Such comparisons can facilitate identification of cis-regulated genes and to establish genetic control of a complex phenotype whose expression is associated with the introgressed chromosome segment. We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated genes during this process. Experiment Overall Design: Experiments were carried out in three batches. In each batch 10 animals per strain were used. After processing each hemisphere separately, 10 hemispheres were pooled for one high-density oligonucleotide microarray (Mouse Genome 430 2.0, Affymetrix, Santa Clara, CA). For each strain 3 oligonucleotide microarrays were used (n=3). We used adult, untreated, male mice (n=30, 90 mice in toto).

ORGANISM(S): Mus musculus

SUBMITTER: Csaba Vadasz

PROVIDER: E-GEOD-7155 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Glutamate receptor metabotropic 7 is cis-regulated in the mouse brain and modulates alcohol drinking.

Vadasz Csaba C Saito Mariko M Gyetvai Beatrix M BM Oros Melinda M Szakall Istvan I Kovacs Krisztina M KM Prasad Vidudala V T S VV Toth Reka R

Genomics 20071023 6

Alcoholism is a heritable disease that afflicts about 8% of the adult population. Its development and symptoms, such as craving, loss of control, physical dependence, and tolerance, have been linked to changes in mesolimbic, mesocortical neurotransmitter systems utilizing biogenic amines, GABA, and glutamate. Identification of genes predisposing to alcoholism, or to alcohol-related behaviors in animal models, has been elusive because of variable interactions of multiple genes with relatively sma ...[more]

PMID: 17936574

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Dataset Information

Transcription profiling of mouse adultbrain hemispheres

Publications

Glutamate receptor metabotropic 7 is cis-regulated in the mouse brain and modulates alcohol drinking.

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