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Unknown,Transcriptomics,Genomics,Proteomics

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RNA-sequencing of cells derived from the site of inflammation of Juvenile Idiopathic Arthritis patients

ABSTRACT: Through RNA sequencing of CD4+ Tmemory/effector cells derived from the synovium of JIA patients and healthy controls, we analyzed the JIA gene expression signature. Treatment of autoinflammatory site-derived patient T cells with the BET-inhibitor JQ1 inhibited immune-related super-enhancers and preferentially reduced disease-associated gene expression, including cytokine-related processes. RNA-sequencing of CD4+ memory/effector T cells derived from Healthy Controls (HC) and JIA patients upon JQ1 treatment.

ORGANISM(S): Homo sapiens

SUBMITTER: Janneke Peeters

PROVIDER: E-GEOD-71595 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Inhibition of Super-Enhancer Activity in Autoinflammatory Site-Derived T Cells Reduces Disease-Associated Gene Expression.

Peeters Janneke G C JG Vervoort Stephin J SJ Tan Sander C SC Mijnheer Gerdien G de Roock Sytze S Vastert Sebastiaan J SJ Nieuwenhuis Edward E S EE van Wijk Femke F Prakken Berent J BJ Creyghton Menno P MP Coffer Paul J PJ Mokry Michal M van Loosdregt Jorg J

Cell reports 20150917 12

The underlying molecular mechanisms for many autoimmune diseases are poorly understood. Juvenile idiopathic arthritis (JIA) is an exceptionally well-suited model for studying autoimmune diseases due to its early onset and the possibility to analyze cells derived from the site of inflammation. Epigenetic profiling, utilizing primary JIA patient-derived cells, can contribute to the understanding of autoimmune diseases. With H3K27ac chromatin immunoprecipitation, we identified a disease-specific, i ...[more]

PMID: 26387944

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Epigenetic profiling of Juvenile Idiopathic Arthritits (JIA) patients

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