Dataset Information

Proteome of neutrophils upon migration towards inflamed synovial fluid

ABSTRACT: Analysis of neutrophil proteomic alterations induced by migration towards inflamed joints in juvenile idiopathic arthritis (JIA). In this experiment neutrophil proteomes were investigated after migration towards JIA synovial fluid in an in vitro model of a synovial membrane, compared to neutrophils incubated in synovial fluid without migration. The migration model consisted of transwell inserts with human knee synoviocytes on the undersides and HMEC endothelial cells on the insides, placed in wells containing medium with 10 % JIA synovial fluid.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Blood

SUBMITTER: Charlotte Welinder

LAB HEAD: Sabine Caroline Arve-Butler

PROVIDER: PXD029129 | Pride | 2022-02-15

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20210706_LFQ_Sabine.mzML	Mzml
	20210706_LFQ_Sabine.mzid.gz	Mzid
	20210706_LFQ_Sabine_090.mzML	Mzml
	20210706_LFQ_Sabine_090.raw	Raw
	20210706_LFQ_Sabine_092.mzML	Mzml

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Publications

Neutrophils Lose the Capacity to Suppress T Cell Proliferation Upon Migration Towards Inflamed Joints in Juvenile Idiopathic Arthritis.

Arve-Butler Sabine S Mossberg Anki A Schmidt Tobias T Welinder Charlotte C Yan Hong H Berthold Elisabet E Król Petra P Kahn Robin R

Frontiers in immunology 20220113

Neutrophils are highly abundant in synovial fluid of rheumatic inflamed joints. In oligoarticular juvenile idiopathic arthritis (JIA), synovial fluid neutrophils have impaired effector functions and altered phenotype. We hypothesized that these alterations might impact the immunoregulatory interplay between neutrophils and T cells. In this study we analyzed the suppressive effect of neutrophils, isolated from blood and synovial fluid of oligoarticular JIA patients, on CD4<sup>+</sup> T cells act ...[more]

PMID: 35095871

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