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Immortalized human hepatocyte (IHH) microRNA response to interferon alpha treatment

ABSTRACT: The purpose of this experiment was to obtain samples for microRNA analysis in IHH cells infected with human interferon alpha. Immortalized human hepatocyte (IHH) cells were seeded at 1 x 10^6 cells per well on 6 well plates 3 days before infection. Treatments were initiated by replacing culture medium (containing 10% FBS) with fresh medium containing 500 U/ml of human interferon α (PBL Biosciences). Mock-treated cells were given normal culture medium only. Mock and treated cells were incubated at 37°C until sample collection time points. Infected samples were collected in triplet; time-matched mocks were collected in triplet in parallel with infected samples. Time points: 6, 12, and 18 h post-infection

ORGANISM(S): Homo sapiens

SUBMITTER: Yoshihiro Kawaoka

PROVIDER: E-GEOD-71733 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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