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Gene expression data from human tonsillar B lymphocytes

ABSTRACT: B lymphocytes are important mediators of adaptive immunity. They drive naÃ¯ve T cells to different T-helper profiles. Th2 responses are implicated in allergic reactions. The goal of this study was to characterize the gene expression profile of B cells under Th2-skewing conditions. B lymphocytes were isolated from human tonsils and cultured for 24 hours under Th2-skewing stimulation (anti-CD40 and IL4 treatment) or with no stimulation. RNA was isolated from 4 biological replicates for each condition. 8 samples in total were hybridized to Affymetrix gene expression microarrays. variable: treatment: antiCD40-IL4: antiCD40-IL4_1, antiCD40-IL4_2, antiCD40-IL4_3, antiCD40-IL4_4 variable: treatment: unstimulated: Control_1, Control_2, Control3, Control_4 repeat: biological replicate: antiCD40-IL4_1, antiCD40-IL4_2, antiCD40-IL4_3, antiCD40-IL4_4 repeat: biological replicate: Control_1, Control_2, Control3, Control_4

ORGANISM(S): Homo sapiens

SUBMITTER: Bruce Mazer

PROVIDER: E-GEOD-71810 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression data from human tonsillar B lymphocytes

Project description:B lymphocytes are important mediators of adaptive immunity. They drive naïve T cells to different T-helper profiles. Th2 responses are implicated in allergic reactions. The goal of this study was to characterize the gene expression profile of B cells under Th2-skewing conditions.

2016-02-15 | GSE71810 | GEO

Transcriptomic analysis of human tonsillar TFH subsets

Project description:We used an IL4-capture assay followed by FACS sorting, to isolate IL4-secreting TFH cells from a human tonsil and compared their transcriptomic profiles with CXCR5hi PD1hi IL4-negative tonsillar TFH cells and IL4-producing CXCR5neg non-TFH cells (TH2 cells). Our studies validate the notion of functionally distinct TFH subsets and identify genes that are specifically expressed in and define the human IL-4 secreting TFH cell subset.

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Synergistic activation by p38MAPK and glucocorticoid signaling mediates induction of M2-like tumor-associated macrophages expressing the novel CD20 homolog MS4A8A

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Synergistic activation by p38MAPK and glucocorticoid signaling mediates induction of M2-like tumor-associated macrophages expressing the novel CD20 homolog MS4A8A

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2011-01-03 | E-GEOD-25707 | biostudies-arrayexpress

Remodeling of Il4-Il13-Il5 locus underlies selective gene expression (fHiC)

Project description:The type 2 cytokines, interleukin (IL)-4, IL-5 and IL-13 reside within a multi-gene cluster in mammals. These cytokines represent the hallmark of type 2 immune responses controlling parasites and promoting tissue repair, as well as causing allergic diseases. Both innate and adaptive lymphocytes secrete type 2 cytokines with discordant production spectra. We took a holistic structural and functional view of the type 2 cytokine locus before and after activation, comparing innate (ILC2) and adaptive (Th2) lymphocytes to understand mechanisms underlying their distinctive programs. Rapid induction of IL-5 dominates in ILC2, whereas IL-4 does so in Th2 cells. Using high-resolution chromatin conformation capture we found that global cellular chromatin architecture remained constant, whereas the type 2 cytokine locus rapidly remodeled. In ILC2, Il13 and Il5 loci were aligned in proximity whereas Il4 locus was insulated. In Th2 cells, Il4 and Il13 positioned in proximity while the Il5 locus remained distal. Select REs were individually deleted in mice to confirm cell-type specific and activation-dependent roles in type 2 responses in vivo. Thus, contrary to the premise that chromatin architecture plays a minimal role in steady-state gene induction, signal-dependent remodeling of 3D configuration underlies the discordant cytokine outputs in ILC2s versus Th2 cells.

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Identification of IL4 dependent genes involved in reinforcement stage of Th2 differentiation

Project description:T helper cells differentiate to many different subclasses depending upon the stimulus and the microenvironment where the cells were stimlated. IL4 is known to polarize the cells towards Th2 differentiation pathway. In this study we aim to identify IL4 dependent genes during late stage of Th2 differentiation.

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