Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Synergistic activation by p38MAPK and glucocorticoid signaling mediates induction of M2-like tumor-associated macrophages expressing the novel CD20 homolog MS4A8A

ABSTRACT: Analysis of bone marrow derived macrophages (BMDM) incubated with dexamethasone&IL4 (Dexa+IL4), B16F1 tumor conditioned medium (cmB16), and B16F1 tumor conditioned medium supplemented with dexamethasone&IL4 (cmB16+dexa+IL4). Results allow detection of genes that require synergistic stimulation of tumor factors and Th2 cytokines. Bone marrow cells were isolated from the tibias and femurs of C57BL/6 mice. M-CSF at a concentration of 30 ng/ml was added to the culture medium [DMEM, 10% FCS, and penicillin/streptomycin]. After 4 days, the culture medium was replaced by fresh DMEM or B16F1 conditioned medium. As indicated, IL-4 (10 ng/ml), dexamethasone (5 × 10−7M), Iwas added. Cells were harvested 3 days later for RNA isolation.

ORGANISM(S): Mus musculus

SUBMITTER: Carsten Sticht 

PROVIDER: E-GEOD-25707 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Synergistic activation by p38MAPK and glucocorticoid signaling mediates induction of M2-like tumor-associated macrophages expressing the novel CD20 homolog MS4A8A.

Schmieder Astrid A   Schledzewski Kai K   Michel Julia J   Tuckermann Jan P JP   Tome Lydia L   Sticht Carsten C   Gkaniatsou Cleopatra C   Nicolay Jan P JP   Demory Alexandra A   Faulhaber Jörg J   Kzhyshkowska Julia J   Géraud Cyrill C   Goerdt Sergij S  

International journal of cancer 20101128 1

Tumor-associated macrophages (TAMs) represent alternatively activated (M2) macrophages that support tumor growth. Previously, we have described a special LYVE-1(+) M2 TAM subset in vitro and in vivo; gene profiling of this TAM subset identified MS4A8A as a novel TAM molecule expressed in vivo by TAM in mammary carcinoma and malignant melanoma. In vitro, Ms4a8a mRNA and MS4A8A protein expression was strongly induced in bone marrow-derived macrophages (BMDMs) by combining M2 mediators (IL-4, gluco  ...[more]

Similar Datasets

Transcriptomics Synergistic activation by p38MAPK and glucocorticoid signaling mediates induction of M2-like tumor-associated macrophages expressing the novel CD20 homolog MS4A8A
2011-01-03 | GSE25707 | GEO
Proteomics Phagosome proteome of activated murine macrophage
2019-03-22 | PXD004520 | Pride
Transcriptomics Bone marrow LysM-Cre mediated TFEB overexpression significantly affected the response of bone marrow-derived macrophages to tumor material, decreasing both the expression of typical M2-like macrophage associated genes and inflammatory response genes.
2020-03-31 | GSE139554 | GEO
Transcriptomics H1 hESC Exposed to Butyrate
2009-03-05 | GSE15110 | GEO
Transcriptomics Single cell RNA sequencing of cultured CCR10+ and CCR10- lung airway epithelial cells
2018-09-04 | GSE115982 | GEO
Transcriptomics The tumor microenvironment shows a hierarchy of cell-cell interactions dominated by fibroblasts [cancer CM]
2023-08-27 | GSE218196 | GEO
Transcriptomics Molecular changes induced by melanoma cell conditioned medium (MCM) in HUVEC cells.
2013-09-30 | E-GEOD-33115 | biostudies-arrayexpress
Transcriptomics Human MCF-7 breast cancer cells: control vs. treated with conditioned medium of cancer associated adipose tissue
2015-01-07 | GSE58574 | GEO
Proteomics Proteins secreted by M1 and M2 macrophages derived from human buffy coat or THP-1 cells
2021-01-21 | MSV000086746 | MassIVE
Transcriptomics Genome-wide analysis of impact of culture conditions on gene expression of primary leukemic cells from chronic lymphocytic leukemia (CLL)
2019-07-14 | GSE80355 | GEO