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Effect of a cardiomyocyte-specific Hira conditional knockout on gene expression in the heart

ABSTRACT: We performed gene expression profiling by microarray using RNA extracted from healthy free wall left ventricle tissues from control and Hira cardiomyocyte-specific conditional knockout mice at 6 weeks of age. Hira is a histone chaperone responsible for replication-independent incorporation of histone variant H3.3 at actively transcribed regions. Conditional knockout of Hira in cardiomyocytes resulted in impaired cardiac function, cardiomyocyte degeneration and focal replacement fibrosis. These results illustrate the role of Hira in controlling the cardiac gene program. 4 animals per group (control and Hira conditional knockout) hybridized in triplicate. RNA was extracted from healthy free wall left ventricle.

ORGANISM(S): Mus musculus

SUBMITTER: M. David Stewart

PROVIDER: E-GEOD-71833 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cardiomyocyte-specific conditional knockout of the histone chaperone HIRA in mice results in hypertrophy, sarcolemmal damage and focal replacement fibrosis.

Valenzuela Nicolas N Fan Qiying Q Fa'ak Faisal F Soibam Benjamin B Nagandla Harika H Liu Yu Y Schwartz Robert J RJ McConnell Bradley K BK Stewart M David MD

Disease models & mechanisms 20160301 3

HIRA is the histone chaperone responsible for replication-independent incorporation of histone variant H3.3 within gene bodies and regulatory regions of actively transcribed genes, and within the bivalent promoter regions of developmentally regulated genes. The HIRA gene lies within the 22q11.2 deletion syndrome critical region; individuals with this syndrome have multiple congenital heart defects. Because terminally differentiated cardiomyocytes have exited the cell cycle, histone variants shou ...[more]

PMID: 26935106

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Effect of a cardiomyocyte-specific Hira conditional knockout on gene expression in the heart

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