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Transcription profiling of rice panicle RNAs were compared with the wild type panicle RNA of similar developmental stage to identify of downstream genes regulated by OsMADS2

ABSTRACT: To gain mechanistic insights on OsMADS2 functions in rice spikelet development, we have employed global expression profilling to identify the genes regulated by OsMADS2. Transgenic rice panicle RNAs were compared with the wild type panicle RNA of similar developmental stage. Genes downregulated in transgenic lines will be those which expression requires functional OsMADS2 whereas upregulated genes will need OsMADS2 for their repression. Experiment Overall Design: Two independent biological RNA pools of dsRNAiOsMADS2 transgenic panicles were compared with a single wild type RNA pool in two different hybridizations. A third hybridization was performed with reciprocally dye-lebeled wild type RNA and one of the mutant RNA.

ORGANISM(S): Oryza sativa

SUBMITTER: Shri Ram Yadav

PROVIDER: E-GEOD-7192 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Divergent regulatory OsMADS2 functions control size, shape and differentiation of the highly derived rice floret second-whorl organ.

Yadav Shri Ram SR Prasad Kalika K Vijayraghavan Usha U

Genetics 20070403 1

Functional diversification of duplicated genes can contribute to the emergence of new organ morphologies. Model eudicot plants like Arabidopsis thaliana and Antirrhinum majus have a single PI/GLO gene that together with AP3/DEF regulate petal and stamen formation. Lodicules of grass flowers are morphologically distinct reduced organs occupying the position of petals in other flowers. They serve a distinct function in partial and transient flower opening to allow stamen emergence and cross-pollin ...[more]

PMID: 17409064

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