Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of human epidermal keratinocytes (NHEK) from neonatal foreskin treated with IL20 subfamily cytokines vs. controls suggests potential roles in cutaneous innate defense and pathogenic adaptive immunity in psoriasis

ABSTRACT: Normal human epidermal keratinocytes (NHEK) from neonatal foreskin were cultured in serum-free EpiLife medium with human KC growth supplement (0.2% bovine pituitary extract (v/v), 5ug bovine insulin, 5ug/ml bovine transferrin, 0.5ng/ml human EGF, and 0.18 ug/ml hydrocortisone) from Cascade Biologics. Cultures were treated with recombinant cytokines from R&D Systems. J Immunol. 2007 Feb 15;178(4):2229-40. Experiment Overall Design: NHEK were treated with IL-19, IL20, IL-22, and IL24, with controls untreated, along with IL1b, IFN gamma, and KGF.

ORGANISM(S): Homo sapiens

SUBMITTER: Kenneth Jung 

PROVIDER: E-GEOD-7216 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

The effects of IL-20 subfamily cytokines on reconstituted human epidermis suggest potential roles in cutaneous innate defense and pathogenic adaptive immunity in psoriasis.

Sa Susan M SM   Valdez Patricia A PA   Wu Jianfeng J   Jung Kenneth K   Zhong Fiona F   Hall Linda L   Kasman Ian I   Winer Jane J   Modrusan Zora Z   Danilenko Dimitry M DM   Ouyang Wenjun W  

Journal of immunology (Baltimore, Md. : 1950) 20070201 4

IL-19, IL-20, IL-22, IL-24, and IL-26 are members of the IL-10 family of cytokines that have been shown to be up-regulated in psoriatic skin. Contrary to IL-10, these cytokines signal using receptor complex R1 subunits that are preferentially expressed on cells of epithelial origin; thus, we henceforth refer to them as the IL-20 subfamily cytokines. In this study, we show that primary human keratinocytes (KCs) express receptors for these cytokines and that IL-19, IL-20, IL-22, and IL-24 induce a  ...[more]

Similar Datasets

Transcriptomics Cytokine treated normal human epidermal keratinocytes
2007-03-09 | GSE7216 | GEO
Transcriptomics SLIGRL-induced gene expression changes in NHEK cells
2019-11-13 | GSE132174 | GEO
Transcriptomics KGF effects on cutaneous SCC cells
2012-05-02 | GSE34652 | GEO
Transcriptomics Control and Il31 tx NHEK cells compared via SuperArray cDNA panel Chemokines and Receptors
2004-04-14 | GSE1304 | GEO
Genomics Adenosine stimulation of normal human epidermal keratinocytes (NHEK)
| PRJNA693696 | ENA
Genomics SLIGRL-induced gene expression changes in NHEK cells
| PRJNA546255 | ENA
Transcriptomics Control and Il31 tx NHEK cells compared via SuperArray cDNA panel Chemokines and Receptors
2010-06-09 | E-GEOD-1304 | biostudies-arrayexpress
Transcriptomics Transcription profiling of human N-Acetyl L-cysteine induced differentiation of NHEK cells in vitro
2005-11-30 | E-MEXP-500 | biostudies-arrayexpress
Transcriptomics Time-dependent global gene expression profile of J774A.1 cells treated with ManLAM in the absence or presence of GW9662
2015-09-02 | GSE72591 | GEO
Transcriptomics Interferon-alpha signaling promotes AGM HSC maturation
2016-07-11 | GSE70878 | GEO