Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Arabidopsis Col-0, ARF10 and mARF10 plants to identify genes regulated by ARF10 in germinating seeds


ABSTRACT: The expression profiles were determined using Affymetrix ATH1 arrays. Comparisons among the Col-0, ARF10 and mARF10 sample groups allow the identification of genes regulated by ARF10. Experiment Overall Design: Germinating seeds (22 hr imbibition) from three independent biological replicates of Col-0, ARF10 and mARF10 samples, which contain three independent seed lots each, were analyzed.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Hiro Nonogaki 

PROVIDER: E-GEOD-7227 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Repression of AUXIN RESPONSE FACTOR10 by microRNA160 is critical for seed germination and post-germination stages.

Liu Po-Pu PP   Montgomery Taiowa A TA   Fahlgren Noah N   Kasschau Kristin D KD   Nonogaki Hiroyuki H   Carrington James C JC  

The Plant journal : for cell and molecular biology 20070802 1


AUXIN RESPONSE FACTORS (ARFs) are transcription factors involved in auxin signal transduction during many stages of plant growth development. ARF10, ARF16 and ARF17 are targeted by microRNA160 (miR160) in Arabidopsis thaliana. Here, we show that negative regulation of ARF10 by miR160 plays important roles in seed germination and post-germination. Transgenic plants expressing an miR160-resistant form of ARF10, which has silent mutations in the miRNA target site (termed mARF10), exhibited developm  ...[more]

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