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Transcription profiling of barley maturing and germinating grains

ABSTRACT: Plant seeds prepare for germination already during seed maturation. We performed a detailed transcriptome analysis of barley grain maturation, desiccation and germination in two tissue fractions (endosperm/aleurone = e/a and embryo = em) using the Affymetrix barley1 chip. Experiment Overall Design: Barley developing and germinating seeds were harvested at different time points after flowering (developing) and imbibition (germinating). To further disseect the influence of different tissues, seeds were dissecte and tissues were analyzed individually.

ORGANISM(S): Hordeum vulgare

SUBMITTER: BjÃ¶rn Usadel

PROVIDER: E-GEOD-9365 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Barley grain maturation and germination: metabolic pathway and regulatory network commonalities and differences highlighted by new MapMan/PageMan profiling tools.

Sreenivasulu Nese N Usadel Björn B Winter Andreas A Radchuk Volodymyr V Scholz Uwe U Stein Nils N Weschke Winfriede W Strickert Marc M Close Timothy J TJ Stitt Mark M Graner Andreas A Wobus Ulrich U

Plant physiology 20080215 4

Plant seeds prepare for germination already during seed maturation. We performed a detailed transcriptome analysis of barley (Hordeum vulgare) grain maturation, desiccation, and germination in two tissue fractions (starchy endosperm/aleurone and embryo/scutellum) using the Affymetrix Barley1 GeneChip. To aid data evaluation, Arabidopsis thaliana MapMan and PageMan tools were adapted to barley. The analyses allow a number of conclusions: (1) Cluster analysis revealed a smooth transition in transc ...[more]

PMID: 18281415

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