Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Response of peripheral blood mononuclear cells to west nile virus (E, NS2a) and type 1 diabetogenic antigens (GAD65, ZnT8) derived peptide stimulation.

ABSTRACT: CD4 T cell responses are characterized based on a limited number of molecular markers selected from exisiting knowledge. The goal of the experiment was to assess antigenic-peptide specific T-cell responses in vitro without bias using microarrays. PBMCs were isolated from 3 healthy donors. The cells were stimulated with peptides derived from west nile virus (E, NS2a), type 1 diabetogenic antigens (GAD65, ZnT8) for 24 hours. Unstimulated cells were cultured without the peptides for 24 hours.

ORGANISM(S): Homo sapiens

SUBMITTER: Scott Presnell

PROVIDER: E-GEOD-72372 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Response of peripheral blood mononuclear cells from 20 healthy donor and 15 patients with type 1 diabetes to type 1 diabetogenic protein (IGRP, PPI) derived peptide stimulation.

Project description:CD4 T cell responses are characterized based on a limited number of molecular markers selected from exisiting knowledge. The goal of the experiment was to assess antigenic-peptide specific T-cell responses in vitro without bias using microarrays. PBMCs were isolated from 20 healthy donors and 15 patients with type 1 diabetes. The cells were stimulated with peptides derived from type 1 diabetogenic protein (IGRP, PPI) for 24 hours. Unstimulated cells were cultured without the peptides for 24 hours.

2016-08-15 | E-GEOD-72377 | biostudies-arrayexpress

Response of peripheral blood mononuclear cells from 15 healthy donor and 15 patients with type 1 diabetes to type 1 diabetogenic protein (GAD65, IGRP, PPI, ZnT8) and influenza virus M derived peptide stimulation.

Project description:CD4 T cell responses are characterized based on a limited number of molecular markers selected from exisiting knowledge. The goal of the experiment was to assess antigenic-peptide specific T-cell responses in vitro without bias using microarrays. PBMCs were isolated from 15 healthy donors and 15 patients with type 1 diabetes. The cells were stimulated with peptides derived from type 1 diabetogenic protein (GAD65, IGRP, PPI, ZnT8) and influenza virus M for 24 hours. Unstimulated cells were cultured without the peptides for 24 hours.

2016-08-15 | E-GEOD-72376 | biostudies-arrayexpress

Response of peripheral blood mononuclear cells from patients with allergy to cat and silver birch wood derived antigenic peptide stimulation.

Project description:CD4 T cell responses are characterized based on a limited number of molecular markers selected from exisiting knowledge. The goal of the experiment was to assess antigenic-peptide specific T-cell responses in vitro without bias using microarrays. PBMCs were isolated from 2 donors with allergy. The cells were stimulated with antigenic peptides derived from silver birch wood and cat for 24 hours. Unstimulated cells were cultured without the peptides for 24 hours.

2016-08-15 | E-GEOD-72373 | biostudies-arrayexpress

Response of peripheral blood mononuclear cells to influenza virus, human cytomegalovirus, and candida albicans derived peptide stimulation.

Project description:CD4 T cell responses are characterized based on a limited number of molecular markers selected from exisiting knowledge. The goal of the experiment was to assess antigenic-peptide specific T-cell responses in vitro without bias using microarrays. PBMCs were isolated from 1 healthy donor. The cells were stimulated with peptides derived from influenza virus H1 and H5, human cytomegalovirus, and candida albicans for 24 hours. Unstimulated cells were cultured without the peptides for 24 hours.

2016-08-15 | E-GEOD-72371 | biostudies-arrayexpress

Response of peripheral blood mononuclear cells to peptide stimuli

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-08-15 | E-GEOD-72605 | biostudies-arrayexpress

Response of peripheral blood mononuclear cells from young (â¤ 45 years old) and old (>60 years old) healthy donors to influenza virus derived peptide stimulation.

Project description:CD4 T cell responses are characterized based on a limited number of molecular markers selected from exisiting knowledge. The goal of the experiment was to assess antigenic-peptide specific T-cell responses in vitro without bias using microarrays. PBMCs were isolated from 11 young (â¤ 45 years old) and old (> 60 years old) healthy donors. The cells were stimulated with peptides derived from influenza virus for 24 hours. Unstimulated cells were cultured without the peptides for 24 hours.

2016-08-15 | E-GEOD-72374 | biostudies-arrayexpress

Response of fresh or frozen peripheral blood mononuclear cells from 1 healthy donor to influenza virus derived peptide stimulation.

Project description:CD4 T cell responses are characterized based on a limited number of molecular markers selected from exisiting knowledge. The goal of the experiment was to assess antigenic-peptide specific T-cell responses in vitro without bias using microarrays. PBMCs were isolated from 1 healthy donor. The fresh cells or cells thawed from cryopresevation (frozen) were stimulated with peptides derived from influenza virus for 24 hours. Unstimulated cells were cultured without the peptides for 24 hours. Experiments were done in triplicate.

2016-08-15 | E-GEOD-72375 | biostudies-arrayexpress

Response of peripheral blood mononuclear cells to west nile virus (E, NS2a) and type 1 diabetogenic antigens (GAD65, ZnT8) derived peptide stimulation.

2016-08-15 | GSE72372 | GEO

Response of peripheral blood mononuclear cells from 20 healthy donor and 15 patients with type 1 diabetes to type 1 diabetogenic protein (IGRP, PPI) derived peptide stimulation.

2016-08-15 | GSE72377 | GEO

2016-08-15 | GSE72376 | GEO