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Transcription profiling of E.coli WT3100 and phoU mutant

ABSTRACT: Comparison of gene experission profiles of Ecoli WT3110 and phoU mutant Experiment Overall Design: DNA microarray analysis of the PhoU mutant with the wild type strain W3110 as a control. The PhoU mutant and the wild type strain were grown in MOPS minimal medium overnight when the RNA was isolated using MasterPure RNA Purification Kit and reverse transcribed for making probes for array hybridization on Affymetrix microarrays.

ORGANISM(S): Escherichia coli

SUBMITTER: Yanqin Yang

PROVIDER: E-GEOD-7243 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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PhoU is a persistence switch involved in persister formation and tolerance to multiple antibiotics and stresses in Escherichia coli.

Li Yongfang Y Zhang Ying Y

Antimicrobial agents and chemotherapy 20070409 6

When a bactericidal antibiotic is added to a growing bacterial culture, the great majority of the bacterial population is killed but a small number of metabolically quiescent bacteria called persisters survive antibiotic treatment. The mechanism of this bacterial persistence is poorly understood. In Escherichia coli, we identified a new persistence gene, phoU, whose inactivation leads to a generalized higher susceptibility than that of the parent strain to a diverse range of antibiotics, includi ...[more]

PMID: 17420206

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