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Carbon utilization in E. coli

ABSTRACT: The study investigated the effect of different carbon sources on E. coli global gene expression. We grew MG1655 cells aerobically in MOPS minimal medium with either glucose, glycerol, succinate, L-alanine, acetate, or L-proline as the carbon supply. Samples were taken from each culture at mid log phase and were RNA-stabilized using Qiagen RNAProtect Bacterial Reagent (Qiagen). Total RNA was then isolated using MasterPure kits (Epicentre Technologies). Purified RNA was reverse-transcribed to cDNA, labeled and hybridized to Affymetrix GeneChipÒ E. coli Antisense Genome Arrays as recommended in the technical manual (www.affymetrix.com). Keywords: parallel sample

ORGANISM(S): Escherichia coli K-12 Escherichia coli

PROVIDER: GSE2037 | GEO | 2005/03/11

SECONDARY ACCESSION(S): PRJNA91865

REPOSITORIES: GEO

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