Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Exogenous isoprene modulates gene expression in unstressed Arabidopsis thaliana plants

ABSTRACT: Isoprene is a well-studied volatile hemiterpene that protects plants from abiotic stress through mechanisms that are not fully understood. The antioxidant and membrane stabilizing potential of isoprene are the two most commonly invoked mechanisms. However, isoprene also affects phenylpropanoid metabolism, suggesting an additional role as a signaling molecule. In this study, microarray based gene expression profiling reveals widespread transcriptional reprogramming of Arabidopsis thaliana plants fumigated for 24 hrs with a physiologically relevant concentration of isoprene. Functional enrichment analysis of fumigated plants revealed enhanced heat- and light-stress-responsive processes in response to isoprene. Isoprene induced a network enriched in ERF and WRKY transcription factors, which may play a role in stress tolerance. The isoprene-induced upregulation of phenylpropanoid biosynthetic genes was specifically confirmed using quantitative reverse transcription polymerase chain reaction. These results support a role for isoprene as a signaling molecule, in addition to its possible roles as an antioxidant and membrane thermoprotectant. Plants were held at 23 °C for 24 hours and then held at 40 °C for 24 hours, either in the presence or absence of 20 PPM isoprene during the entire 48 hours. Leaf samples were taken at the end of both 24 hour treatment periods. Each of the 4 resulting conditions was replicated 3 times.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Thomas Sharkey

PROVIDER: E-GEOD-72547 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Exogenous isoprene modulates gene expression in unstressed Arabidopsis thaliana plants

Project description:Isoprene is a well-studied volatile hemiterpene that protects plants from abiotic stress through mechanisms that are not fully understood. The antioxidant and membrane stabilizing potential of isoprene are the two most commonly invoked mechanisms. However, isoprene also affects phenylpropanoid metabolism, suggesting an additional role as a signaling molecule. In this study, microarray based gene expression profiling reveals widespread transcriptional reprogramming of Arabidopsis thaliana plants fumigated for 24 hrs with a physiologically relevant concentration of isoprene. Functional enrichment analysis of fumigated plants revealed enhanced heat- and light-stress-responsive processes in response to isoprene. Isoprene induced a network enriched in ERF and WRKY transcription factors, which may play a role in stress tolerance. The isoprene-induced upregulation of phenylpropanoid biosynthetic genes was specifically confirmed using quantitative reverse transcription polymerase chain reaction. These results support a role for isoprene as a signaling molecule, in addition to its possible roles as an antioxidant and membrane thermoprotectant.

2015-09-01 | GSE72547 | GEO

Microarray analysis for leaves of Arabidopsis thaliana Col-0 plants fumigated with nitrogen dioxide (treatment) or air (control)

Project description:Four-week-old Arabidopsis thaliana (Col-0) plants were fumigated for 1 h with 10 parts per million (ppm) nitrogen dioxide (NO2) to analyse leaf transcriptome changes induced by this air pollutant relative to air-fumigated control plants.

2018-08-10 | E-MTAB-6522 | biostudies-arrayexpress

Transcription profiling of Arabidopsis seedlings treated with the herbicide methyl viologen

Project description:We exposed plants to methyl viologen (MV), a redox cycling herbicide in the light, and perform biochemical and Affymetrix ATH1 GeneChip experiments under conditions in which photosynthesis was active and the antioxidant response well conserved. Samples of total RNA were obtained from a pool of 25 Arabidopsis specimens from two independent biological replicates from the aerial parts of 2h MV-treated and non-treated control seedlings.

2008-01-08 | E-ATMX-28 | biostudies-arrayexpress

Comparative Analysis of Seed Transcriptomes of Ambient Ozone-Fumigated Two Different Rice Cultivars

Project description:High ozone (O3) concentration causes serious damages in plant productivity. Climate models forecast that ground O3 level in the future will reach phytotoxic range, resulting in crop yield losses. With an ultimate goal to screen molecular factors to minimize losses of crop production by the rise of O3 level, we have started an investigation on effects of O3 on rice using rice DNA chip. Herein, we have utilized the samples of dry mature rice seeds harvested in an ozone-sensitive rice cultivar (Oryza sativa L. indica cv. Takanari) and a tolerant cultivar (Oryza sativa L. japonica cv. Koshihikari) which were fumigated with ambient air (mean O3: 32.7 ppb) in small open-top chambers (OTCs). First, we extracted total RNA from dry mature rice seeds of Takanari and Koshihikari using a modified protocol based on cethyltrimethylammonium bromide extraction buffer and phenol-chloroform-isoamylalcohol treatment. Furthermore, to perform microarray analysis using the Agilent 4x44 rice DNA Chip and the dye-swap method, we designed a balanced block design comparing seeds in an ambient air-fumigated rice cultivar and those in a filtered air-fumigated rice cultivar. Direct comparison of Koshihikari and Takanari O3 transcriptomes in seeds of rice plants fumigated with ambient O3 in OTCs successfully showed that genes encoding proteins involved in jasmonic acid, GABA biosynthesis, cell wall and membrane modification, starch mobilization, and secondary metabolite biosynthesis are differently regulated in an O3-sensitive cv. Takanari and a tolerant cv. Koshihikari. Comparison between O. sativa L. indica cv. Takanari and japonica cv. Koshihikari grown under ozone for their lifetime was performed. Controls were plants grown under filtered air. Three biological replicates (4 plants in each biological replicate in each small open top chamber - seed; pooled) were used, and dye-swaped.

2013-08-20 | E-GEOD-49963 | biostudies-arrayexpress

Oryza sativa

Project description:Microarray analysis of rice plants fumigated with ozone

| PRJNA106843 | ENA

Transcriptomic profiling of rice (Oryza sativa) cv. Sijung and Jumli Marshi during early chilling stress

Project description:The aim of this study was to conduct global gene expression profiling and comparative analysis of two chilling tolerant rice varieties, Jumli Marshi and Sijung (spp. Japonica), during early chilling stress (24h, 10C). Leaf tissue from cv. Jumli Marshi and cv. Sijung plants grown under chilling stress conditions were harvested after 4 and 24 h. Control plants (0 h) were harvested at the beginning of the experiment, i.e., at mid-day. Three biological replicates were profiled for each time point and variety. In order to readily detect highly (saturated) as well as weakly (near background signal) expressed genes, scanning was done twice on the microarrays: at the PMT sensitivity level 100% (pmt100) and 10% (pmt10).

2014-11-30 | E-MTAB-3121 | biostudies-arrayexpress

Expression data from micropropagated Vitis vinifera when transferred to ex vitro conditions

Project description:Oxidative stress can arise when in vitro propagated plants developed under low light conditions are exposed to high light during transfer to ex vitro conditions. In such a situation, among the many potential stresses to which the transferred plant can be exposed, oxidative stress is commonly experienced, most likely brought about by absorption of light energy in excess of that required for very low levels of photosynthetic metabolism. In vitro propagated grapevine when transferred to ex vitro conditions with a 4 fold increase in PPFD shows an initial inhibition of PET accompanied by an accumulation of H2O2, suggesting a signal for the upregulation in gene expression and antioxidant enzyme activity, which peaked at 48h after transfer of in vitro grapevine to ex vitro growing conditions. When in vitro propagated plants are exposed to high light upon transfer to ex vitro oxidative stress symptoms occur. To determine whether the underlying pathways activated at the transfer of in vitro grapevine to ex vitro conditions reflect the processes occurring upon light stress we used microarrays. Leaves were harvested from in vitro grown plants immediately prior to transfer to ex vitro condition and 48h after transfer to compare gene expression before and after exposure of these plants to the high light conditions typical of ex vitro growth.

2011-02-10 | E-GEOD-27180 | biostudies-arrayexpress

Comparative Analysis of Seed Transcriptomes of Ambient Ozone-Fumigated Two Different Rice Cultivars

2012-04-30 | E-GEOD-37650 | biostudies-arrayexpress

Comparative Analysis of Seed Transcriptomes of Ambient Ozone-Fumigated Two Different Rice Cultivars

2012-05-01 | GSE37650 | GEO

Comparative Analysis of Seed Transcriptomes of Ambient Ozone-Fumigated Two Different Rice Cultivars

2013-08-20 | GSE49963 | GEO