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A spectral analysis approach to detects actively translated open reading frames in high resolution ribosome profiling data

ABSTRACT: RNA sequencing protocols allow for quantifying gene expression regulation at each individual step, from transcription to protein synthesis. Ribosome Profiling (Ribo-seq) maps the positions of translating ribosomes over the entire transcriptome. Despite its great potential, a rigorous statistical approach to identify translated regions from Ribo-seq data is not yet available. To fill this gap, we developed RiboTaper, which quantifies the significance of the three-nucleotide periodicity of Ribo-seq reads via spectral analysis methods. Examination of RNA fragments protected by ribosome

ORGANISM(S): Homo sapiens

SUBMITTER: Neelanjan Mukherjee

PROVIDER: E-GEOD-73136 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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