Unknown,Transcriptomics,Genomics,Proteomics

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Expression analysis of CsMYBF1 overexpressing tomato fruit

ABSTRACT: To investigate global genome expression changes caused by the ectopic expression of CsMYBF1, we compared the gene expression profiles of the transgenic and wild type tomato fruits by RNA-seq analysis.Fruit samples from at least five plants of each genotype, which were harvested between 7 and 10 days after breaker stage, were pooled for total RNA isolation.A total of 3296 genes were differentially expressed between the transgenic and wild type fruits, and most of them were significantly induced, indicating the widespread influence on the expression of the endogenous tomato genes. Two lines of the tomato transgenic fruits expressing citrus gene CsMYBF1 were compared with two of that from the wild type.

ORGANISM(S): Solanum lycopersicum

SUBMITTER: chaoyang Liu

PROVIDER: E-GEOD-73300 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Fruits of transgenic tomato (Solanum lycopersicum) plants engineered with ripening-induced, yeast S-adenosylmethionine decarboxylase (ySAMdc) gene, accumulate the higher polyamines spermidine (Spd) and spermine (Spm) and demonstrate ameliorated phytonutrient content, juice quality, and prolonged vine life. Enhanced nitrogen-carbon interactions were revealed by comprehensive Nuclear Magnetic Resonance (NMR) spectroscopy-based metabolite profiling of the transgenics, suggesting that Spd and Spm are perceived as nitrogenous metabolites by the fruit cells (Mattoo et al., 2006). The recent work by our colleagues identified the effects of Spd/Spm accumulation on various functional classes of tomato genes affected during ripening by probing 1522 ESTs on a custom-made array (Srivastava et al., 2007). In this study we monitored alterations of genome-wide transcriptional patterns in pericarp of Spd/Spm-accumulating tomatoes by means of direct comparison with azygous controls using DNA-microarray technology. Consistent with the ySAMdc expression pattern, very minor transcriptional alterations were detected in mature green developmental stage. For both breaker and red stages, large mutual and unique gene sets displayed altered levels of transcript. Ontological term analysis of up- and down-regulated transcript groups revealed processes in cell metabolism that are regulated by increased levels of Spd/Spm in ripening tomato fruits. These processes mainly involve carbohydrate and amino acids metabolism and protein synthesis. Additionally, transcript levels of representative genes encoding structural enzymes for related biosynthetic pathways show strong relationship to specific metabolites that were identified as regulated in Spd/Spm-accumulating transgenics. Plants of both genotypes were grown in randomized blocks (3 blockes 8-10 plants each). At each of 3 developmental stages (mature green, breaker and pink-red) a single fruit was harvested from a randomly chosen plant of each genotype within each block. In each harvest different plants in each block were taken to represent each genotype. Following harvest, equal pericarp samples were cut from the equator region of each fruit. These samples were pooled according to genotype prior to RNA extraction, allowing equal representation of each block in the final sample. A total of 3 harvests taken from each genotype were eventually analyzed– each harvest representing a biological repeat.

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Expression analysis of CsMYBF1 overexpressing tomato fruit

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