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Transcription profiling of S. cerevisiae H2A4-20 mutants vs. wild types

ABSTRACT: Total RNA from three replicate cultures of wild-type and mutant strains was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by histone H2A^4-20 mutant. Experiment Overall Design: Comparison of genes whose mRNA levels are affected in a histone H2A^4-20 mutant yeast strain compared to wild-type yeast strain.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: John Wyrick

PROVIDER: E-GEOD-7337 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Regulation of gene transcription by the histone H2A N-terminal domain.

Parra Michael A MA Wyrick John J JJ

Molecular and cellular biology 20070827 21

Histone N-terminal domains play critical roles in regulating chromatin structure and gene transcription. Relatively little is known, however, about the role of the histone H2A N-terminal domain in transcription regulation. We have used DNA microarrays to characterize the changes in genome-wide expression caused by mutations in the N-terminal domain of histone H2A. Our results indicate that the N-terminal domain of histone H2A functions primarily to repress the transcription of a large subset of ...[more]

PMID: 17724083

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