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Transcription profiling of S. cerevisiae Histone H2A K4,7G mutant vs. wild type

ABSTRACT: Total RNA from three replicate cultures of wild-type and mutant strains was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by the histone H2A K4,7G mutant. Experiment Overall Design: Comparison of genes whose mRNA levels are affected in a histone H2A K4,7G mutant yeast strain compared to wild-type yeast strain.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: John Wyrick

PROVIDER: E-GEOD-7338 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Regulation of gene transcription by the histone H2A N-terminal domain.

Parra Michael A MA Wyrick John J JJ

Molecular and cellular biology 20070827 21

Histone N-terminal domains play critical roles in regulating chromatin structure and gene transcription. Relatively little is known, however, about the role of the histone H2A N-terminal domain in transcription regulation. We have used DNA microarrays to characterize the changes in genome-wide expression caused by mutations in the N-terminal domain of histone H2A. Our results indicate that the N-terminal domain of histone H2A functions primarily to repress the transcription of a large subset of ...[more]

PMID: 17724083

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