Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of S. cerevisiae Histone H2A K4,7G mutant vs. wild type

ABSTRACT: Total RNA from three replicate cultures of wild-type and mutant strains was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by the histone H2A K4,7G mutant. Experiment Overall Design: Comparison of genes whose mRNA levels are affected in a histone H2A K4,7G mutant yeast strain compared to wild-type yeast strain.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: John Wyrick

PROVIDER: E-GEOD-7338 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Regulation of gene transcription by the histone H2A N-terminal domain.

Parra Michael A MA Wyrick John J JJ

Molecular and cellular biology 20070827 21

Histone N-terminal domains play critical roles in regulating chromatin structure and gene transcription. Relatively little is known, however, about the role of the histone H2A N-terminal domain in transcription regulation. We have used DNA microarrays to characterize the changes in genome-wide expression caused by mutations in the N-terminal domain of histone H2A. Our results indicate that the N-terminal domain of histone H2A functions primarily to repress the transcription of a large subset of ...[more]

PMID: 17724083

Similar Datasets

Transcription profiling of S. cerevisiae H2A4-20 mutants vs. wild types

Project description:Total RNA from three replicate cultures of wild-type and mutant strains was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by histone H2A^4-20 mutant. Experiment Overall Design: Comparison of genes whose mRNA levels are affected in a histone H2A^4-20 mutant yeast strain compared to wild-type yeast strain.

2008-06-15 | E-GEOD-7337 | biostudies-arrayexpress

Expression data from Saccharomyces cerevisiae histone H2A mutants

Project description:The N-terminal tail of histone H2A shows evolutionary changes that parallel genome size and aid chromatin compaction. As genome size increases, so does the number of arginines. In contrast, serines corellate with small genomes. Examples for such changes are arginine in position 11 and serine in position 15. To test if these residues affect mRNA levels, we analysed gene expression profiles of S.cerevisiae strains containing either WT or mutant H2A. Yeast strains have endogenous histone H2A genes deleted and express plasmid born WT or mutant H2A. PolyA RNA of these strains was analyzed by single channel microarray hybridization. Three WT biological replicates provide the control. Two biological replicates of each of the mutants containing either R11, K11, carrying a deletion in S15 and the double mutant carrying both the deletion of S15 and an insertion of R11 are analysed.

2014-03-01 | E-GEOD-50440 | biostudies-arrayexpress

Gene expression changes in a histone sprocket arginine mutant in histone H2A R78A

Project description:Examined gene expression changes in a histone H2A R78A mutant in Saccharomyces cerevisiae relative to wild-type cells. THe overall goal of this study was to determine the functions of histone 'sprocket' arginine residues, which insert into the DNA minor groove in the nucleosome. We examined the roles of sprocket arginine mutants in gene expression, histone incorporation, and DNA repair. Three wild-type control replicate samples and three experimental (H2A R78A) replicates

2015-08-05 | E-GEOD-68159 | biostudies-arrayexpress

Histone H2A K4,7G

Project description:Total RNA from three replicate cultures of wild-type and mutant strains was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by the histone H2A K4,7G mutant. Keywords: histone H2A mutant, repeat

2007-10-11 | GSE7338 | GEO

Histone H2A^4-20

2007-10-11 | GSE7337 | GEO

Transcription profiling of S. cerevisiae Histone H3 K4,79R mutant vs. wild type

Project description:Total RNA from three replicate cultures of wild-type and mutant strains was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H3 K4,79R mutant. Experiment Overall Design: Comparison of genes whose mRNA levels are affected in a histone H3 K4,79R mutant yeast strain compared to wild-type yeast strain.

2008-06-14 | E-GEOD-6319 | biostudies-arrayexpress

Transcription profiling of S. cerevisiae mutant and wild type strains to identify genes regulated by the histone H3 K4,9,14,18,23,27,36,79G mutant

Project description:Total RNA from two replicate cultures of wild-type and mutant strains was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by histone H3 K4,9,14,18,23,27,36,79G mutant. Experiment Overall Design: Comparison of genes whose mRNA levels are affected in a histone H3 K4,9,14,18,23,27,36,79G mutant yeast strain compared to wild-type yeast strain.

2009-01-14 | E-GEOD-10933 | biostudies-arrayexpress

Expression data from Saccharomyces cerevisiae histone H2A mutants

2014-03-01 | GSE50440 | GEO

Transcription profiling of S. cerevisiae strain with histone H4 R45 mutated to H with wild type yeast strain WY139

Project description:Total RNA samples from three replicate cultures of wild type and mutant yeast strains was isolated and expression profile done using Affymetrix arrays. Comparsion between the samples indicate how mutation in a single amino acid residue in histone H4 (H4R45H) affects gene expression in yeast. Such a mutation in histone H4 is known to generate a specific class of mutants called SWI/SNF independent (SIN) mutants, and the mutants were identified by their ability to carry out transcription in the absence of yeast chromatin remodeling complex SWI/SNF. SIN mutations are known to affect higher order chromatin structure and the comparative expression profile would help identification of genes which get affected by such altered chromatin landscape. Experiment Overall Design: Comparison of genes whose mRNA levels are affected in histone H4R45H mutant yeast strains compared to wild type yeast strain WY139. This comparison reveals how subtle change in a single amino acid residue in core domain of histone H4 affects gene expression profile in yeast.

2008-10-20 | E-GEOD-11282 | biostudies-arrayexpress

Glutamine methylation on Histone H2A is an RNA Polymerase I dedicated modification

Project description:Nucleosomes are decorated with numerous post-translational modifications capable of influencing many DNA processes. Here, we describe a new class of modification, methylation of glutamine, occurring on yeast histone H2A at position 105 (Q105) and human H2A at Q104. We identify Nop1 as the methyltransferase in yeast and we demonstrate that Fibrillarin is the equivalent enzyme in human cells. Glutamine methylation of H2A is restricted to the nucleolus. Global analysis in yeast, using a H2AQ105me specific antibody, show that this modification is exclusively enriched over the 35S rDNA transcriptional unit. We show that the Q105 residue is part of the binding site for the histone chaperone FACT (Facilitator of Transcription) complex. Methylation of Q105 or its substitution to alanine disrupts binding to FACT in vitro. A yeast strain mutated at Q105 exhibits a defect in histone incorporation and shows increased transcription at rDNA genes. This defect is phenocopied by mutations in FACT that decrease its activity. Together these data identify glutamine methylation of H2A as the first histone epigenetic mark dedicated to a specific RNA polymerase and define its function as a regulator of FACT interaction with nucleosomes.

2014-01-21 | E-MTAB-1447 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data