Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Arabidopsis mutant tdt-1 and wild type


ABSTRACT: The object of this study was to explore whether the loss of mRNA decapping in the Arabidopsis mutant tdt-1 resulted in global dfferences of RNA profiles, as compared to wild type. Experiment Overall Design: In this experiment we compared expression in tdt-1 and wild type 3 day whole seedlings. We performed three biological replicates, in which one experiment was dye-swapped.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: David Goeres 

PROVIDER: E-GEOD-7359 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Components of the Arabidopsis mRNA decapping complex are required for early seedling development.

Goeres David C DC   Van Norman Jaimie M JM   Zhang Weiping W   Fauver Nellie A NA   Spencer Mary Lou ML   Sieburth Leslie E LE  

The Plant cell 20070518 5


To understand the mechanisms controlling vein patterning in Arabidopsis thaliana, we analyzed two phenotypically similar mutants, varicose (vcs) and trident (tdt). We had previously identified VCS, and recently, human VCS was shown to function in mRNA decapping. Here, we report that TDT encodes the mRNA-decapping enzyme. VCS and TDT function together in small cytoplasmic foci that appear to be processing bodies. To understand the developmental requirements for mRNA decapping, we characterized th  ...[more]

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