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RNA sequencing of e15.5 pancreas from Wild Type, Blinc1-/- and Blinc+/- mice.

ABSTRACT: We report the transcriptome changes that result of the genomic deletion of one or two alleles of an islet-specific long non-coding RNA (Blinc1) in isolated pancreas from e15.5 mouse embryos. Pancreas from e15.5 embryos were dissected and total RNA extracted. Libraries were prepared from total RNA (RIN>8) with the TruSeq RNA prep kit (Illumina) and sequenced using the HiSeq2000 (Illumina) instrument. More than 20 million reads were mapped to the mouse genome (UCSC/mm9) using Tophat (version 2.0.4) with 4 mismatches and 10 maximum multiple hits. Significantly differentially expressed genes were calculated using DEseq.

ORGANISM(S): Mus musculus

SUBMITTER: Lori Sussel

PROVIDER: E-GEOD-73711 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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βlinc1 encodes a long noncoding RNA that regulates islet β-cell formation and function.

Arnes Luis L Akerman Ildem I Balderes Dina A DA Ferrer Jorge J Sussel Lori L

Genes & development 20160301 5

Pancreatic β cells are responsible for maintaining glucose homeostasis; their absence or malfunction results in diabetes mellitus. Although there is evidence that long noncoding RNAs (lncRNAs) play important roles in development and disease, none have been investigated in vivo in the context of pancreas development. In this study, we demonstrate that βlinc1 (β-cell long intergenic noncoding RNA 1), a conserved lncRNA, is necessary for the specification and function of insulin-producing β cells t ...[more]

PMID: 26944677

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