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Single-cell transcriptomics reveals unique features of human pancreatic islet cell subtypes

ABSTRACT: We report the single-cell RNA-seq based identification of 6 known human islet cell types (alpha cells, beta cells, delta cells, pp cells, acinar cells and duct cells) based on the expression of known marker genes. We further assess cell type specific gene expression and suggest novel marker genes for several cell types. Transcriptional dissection of human pancreatic islets of one donor using single-cell RNA-seq

ORGANISM(S): Homo sapiens

SUBMITTER: Christoph Bock

PROVIDER: E-GEOD-73727 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Single-cell transcriptomes reveal characteristic features of human pancreatic islet cell types.

Li Jin J Klughammer Johanna J Farlik Matthias M Penz Thomas T Spittler Andreas A Barbieux Charlotte C Berishvili Ekaterine E Bock Christoph C Kubicek Stefan S

EMBO reports 20151221 2

Pancreatic islets of Langerhans contain several specialized endocrine cell types, which are commonly identified by the expression of single marker genes. However, the established marker genes cannot capture the complete spectrum of cellular heterogeneity in human pancreatic islets, and existing bulk transcriptome datasets provide averages across several cell populations. To dissect the cellular composition of the human pancreatic islet and to establish transcriptomes for all major cell types, we ...[more]

PMID: 26691212

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