Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse IgM+IgD+ cells and srf-deficient IgM+IgD+ cells isolated from spleens to investigate the role of serum response factor


ABSTRACT: Serum response factor (SRF), a MADS-box transcription factor, is essential for murine embryonic development and for the function of muscle cells and neurons. SRF and its transcriptional co-factors are broadly expressed. To determine the in vivo role of SRF in developing lymphocytes we specifically inactivated the murine Srf gene during T or B cell development using lymphocyte-specific Cre transgenic mouse lines. T cell-specific Srf deletion led to a severe block in thymocyte development at the transition from double to single positive stage. The few residual T cells detectable in the periphery retained at least one functional Srf allele, thereby demonstrating the importance of SRF in T cell development. In contrast, deletion of Srf in developing B cells did not interfere with the growth and survival of B cells in general, yet led to a complete loss of marginal zone B cells and a marked reduction of the CD5+ B cell subset. Our study also revealed a contribution of SRF to the expression of the surface molecules IgM, CD19, and the chemokine receptor 4 in B lymphocytes. Experiment Overall Design: Comparison of the gene expression profile between murine wildtype IgM+IgD+ cells and srf-deficient IgM+IgD+ cells isolated from spleens

ORGANISM(S): Mus musculus

SUBMITTER: Robert Geffers 

PROVIDER: E-GEOD-7412 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Serum response factor contributes selectively to lymphocyte development.

Fleige Anne A   Alberti Siegfried S   Gröbe Lothar L   Frischmann Ursula U   Geffers Robert R   Müller Werner W   Nordheim Alfred A   Schippers Angela A  

The Journal of biological chemistry 20070625 33


Serum response factor (SRF), is a crucial transcription factor for murine embryonic development and for the function of muscle cells and neurons. Gene expression data show that SRF and its transcriptional cofactors are also expressed in lymphocyte precursors and mature lymphocytes. However, the role of SRF in lymphocyte development has not been addressed in vivo so far, attributed in part to early embryonic lethality of conventional Srf-null mice. To determine the in vivo role of SRF in developi  ...[more]

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