Real-time quantitative PCR analysis of mice lung tissue [S100A9 time course]
Ontology highlight
ABSTRACT: Recombinant-murine S100A9 were used at 10 mg/50 ml in Hanks Balanced Salt solution (HBSS) or control HBSS administered onto the nares of BALB/C mice. To assess direct effects, mice were sacrificed 1, 4, 6, or 12 h post-inhalation of S100A9. Because S100A9 is reported to initiate proinflammatory responses by ligating TLR4 and/or RAGE, a quantitative PCR array was developed to analyze 49 genes, selected to reflect potential acute inflammatory changes induced by ligation of these receptors. Expression of inflammatory genes was evaluated with the RT-qPCR array. Relative quantities of mRNA in duplicate samples were obtained using the LightCycler® 480 Software 1.5 and the Efficiency-Method.
ORGANISM(S): Mus musculus
SUBMITTER: Kenneth Hsu
PROVIDER: E-GEOD-74128 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
ACCESS DATA