Unknown,Transcriptomics,Genomics,Proteomics

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Real-time quantitative PCR analysis of mice lung tissue (2)


ABSTRACT: Recombinant-murine S100A8 and the corresponding Cys42 to Ala42 mutant were used at 10 mg/50 ml HBSS administered onto the nares of BALB/C mice. To assess direct effects, mice were sacrificed 1, 4, 6, 12 or 20 h post-inhalation of S100A8 or 12 and 20 h post-inhalation of Ala42S100A8. For comparison with Dex inhalation (used at 10 mg/50 ml HBSS), lungs were harvested 6 and 12 h post administration. Because S100A8 is reported to initiate proinflammatory responses by ligating TLR4 and/or RAGE, a quantitative PCR array was developed to analyse 63 genes, selected to reflect potential acute inflammatory changes induced by ligation of these receptors. Expression of inflammatory genes was evaluated with the RT-qPCR array. Relative quantities of mRNA in duplicate samples were obtained using the LightCyclerM-BM-. 480 Software 1.5 and the Efficiency-Method.

ORGANISM(S): Mus musculus

SUBMITTER: Kenneth Hsu 

PROVIDER: E-GEOD-52719 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

S100A8 induces IL-10 and protects against acute lung injury.

Hiroshima Yuka Y   Hsu Kenneth K   Tedla Nicodemus N   Chung Yuen Ming YM   Chow Sharron S   Herbert Cristan C   Geczy Carolyn L CL  

Journal of immunology (Baltimore, Md. : 1950) 20140214 6


S100A8 is considered proinflammatory by activating TLR4 and/or the receptor for advanced glycation end products. The aim was to investigate inflammatory effects of S100A8 in murine lung. S100A8 was administered to BALB/c mice by nasal inhalation and genes induced over a time-course assessed. LPS was introduced intranasally either alone or 2 h after pretreatment of mice with intranasal application of S100A8 or dexamethasone. A Cys(42)-Ala(42) mutant S100A8 mutant was used to assess whether S100A8  ...[more]

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