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Ribosome hibernation factor promotes Staphyloccocal survival and differentially represses translation

ABSTRACT: We sequenced a total of 16 cDNA libararies derived from fragmented total mRNA and ribosome protected mRNAs from S. aureus hpf mutant (NE838) and its parental strain JE2 grown in tryptic soy broth (TSB) or minimal medium (MM). The data represented 2 independent biological replicates. Examination of the impact of hpf on global S.aureus translatome, mRNA abundance and the ribosome density along the transcripts.

ORGANISM(S): Staphylococcus aureus

SUBMITTER: Mee-Ngan Yap

PROVIDER: E-GEOD-74197 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Ribosome hibernation factor promotes Staphylococcal survival and differentially represses translation.

Basu Arnab A Yap Mee-Ngan F MN

Nucleic acids research 20160321 10

In opportunistic Gram-positive Staphylococcus aureus, a small protein called hibernation-promoting factor (HPFSa) is sufficient to dimerize 2.5-MDa 70S ribosomes into a translationally inactive 100S complex. Although the 100S dimer is observed in only the stationary phase in Gram-negative gammaproteobacteria, it is ubiquitous throughout all growth phases in S. aureus The biological significance of the 100S ribosome is poorly understood. Here, we reveal an important role of HPFSa in preserving ri ...[more]

PMID: 27001516

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