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Overexpression of PHF8 promotes an EMT-related gene signature in MCF10A cells

ABSTRACT: PHF8 exerts distinct functions in different types of cancer. However, the mechanisms underlying its specific functions in each case remain obscure. To establish whether overexpression of PHF8 regulates the TGF-Î² induced the epithelial-mesenchymal transition (EMT), we treated MCF10A-Mock (control) and MCF10A-PHF8wt (overexpressing wild-type PHF8) cells with TGF-Î²1 for 0, 24, 48 and 72 hours and performed RNA-seq in biological duplicates. Our data indicated that EMT gene signatures were significantly enriched in MCF10A-PHF8 cells with TGF-Î²1 treatment at all time points, strongly indicating that PHF8 overexpression induces a sustained EMT signaling program. mRNA profiles of MCF10A-Mock (control) and MCF10A-PHF8 with TGF-Î²1 treatment for 0, 24, 48 and 72 hours were generated by RNA-seq, in duplicate, using HiSeq2500 instrument.

ORGANISM(S): Homo sapiens

SUBMITTER: Hank Qi

PROVIDER: E-GEOD-74377 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Homo sapiens

Project description:Overexpression of PHF8 promotes an EMT-related gene signature in MCF10A cells

| PRJNA299891 | ENA