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Overexpression of PHF8 promotes an EMT-related gene signature in MCF10A cells

ABSTRACT: PHF8 exerts distinct functions in different types of cancer. However, the mechanisms underlying its specific functions in each case remain obscure. To establish whether overexpression of PHF8 regulates the TGF-β induced the epithelial-mesenchymal transition (EMT), we treated MCF10A-Mock (control) and MCF10A-PHF8wt (overexpressing wild-type PHF8) cells with TGF-β1 for 0, 24, 48 and 72 hours and performed RNA-seq in biological duplicates. Our data indicated that EMT gene signatures were significantly enriched in MCF10A-PHF8 cells with TGF-β1 treatment at all time points, strongly indicating that PHF8 overexpression induces a sustained EMT signaling program.

ORGANISM(S): Homo sapiens

PROVIDER: GSE74377 | GEO | 2016/10/26

SECONDARY ACCESSION(S): PRJNA299891

REPOSITORIES: GEO

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Overexpression of PHF8 promotes an EMT-related gene signature in MCF10A cells

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