Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptomic analysis of Grapevine (cv. Summer Black) leaf at four developmental stages, using the Illumina Platform


ABSTRACT: The present study was designed to find out the transcriptional system that is connected with the development of leaves and production of metabolites during photosynthesis. We examine the cellular expression by use the up-to-date RNA sequencing expertise to carry out a complete analysis of the transcriptional profile of V. vinifera leaves at four different developmental stages. Total RNA extracted from 4 samples with three replication for each and compared within all samples.

ORGANISM(S): Vitis vinifera

SUBMITTER: Tariq Pervaiz 

PROVIDER: E-GEOD-74428 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptomic Analysis of Grapevine (cv. Summer Black) Leaf, Using the Illumina Platform.

Pervaiz Tariq T   Haifeng Jia J   Salman Haider Muhammad M   Cheng Zhang Z   Cui Mengjie M   Wang Mengqi M   Cui Liwen L   Wang Xicheng X   Fang Jinggui J  

PloS one 20160129 1


Proceeding to illumina sequencing, determining RNA integrity numbers for poly RNA were separated from each of the four developmental stages of cv. Summer Black leaves by using Illumina HiSeq™ 2000. The sums of 272,941,656 reads were generated from vitis vinifera leaf at four different developmental stages, with more than 27 billion nucleotides of the sequence data. At each growth stage, RNA samples were indexed through unique nucleic acid identifiers and sequenced. KEGG annotation results depict  ...[more]

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