Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proinflammatory isoforms of IL-32 as novel and robust biomarkers for control failure in HIV-infected slow-progressors.

ABSTRACT: HIV-infected slow-progressors (SP) represent a heterogeneous group of subjects who spontaneously control HIV infection without treatment for several years while showing moderate signs of disease progression. Here we determined the frequency of SP subjects who showed loss of HIV control within our Canadian Cohort of HIV+ Slow-Progressors and identified the proinflammatory cytokine IL-32 as a robust biomarker for control failure. Total cellular RNA was extracted from PBMC from 5 donors at two time points ('V1' before and 'V2' after loss of control, and only Long Term Non Progressors (LTNP) were part of the case study), along with 2 control Leuka914 and Aging106032 samples.

ORGANISM(S): Homo sapiens

SUBMITTER: Jean-Philippe Goulet

PROVIDER: E-GEOD-74790 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Proinflammatory isoforms of IL-32 as novel and robust biomarkers for control failure in HIV-infected slow-progressors.

Project description:HIV-infected slow-progressors (SP) represent a heterogeneous group of subjects who spontaneously control HIV infection without treatment for several years while showing moderate signs of disease progression. Here we determined the frequency of SP subjects who showed loss of HIV control within our Canadian Cohort of HIV+ Slow-Progressors and identified the proinflammatory cytokine IL-32 as a robust biomarker for control failure.

2015-12-31 | GSE74790 | GEO

RNA-seq of HIV-1 infected long-term non-progressors(LTNPs) and typical progressors(TPs)

Project description:HIV-infected long-term non-progressors (LTNPs) are a special group of people who can naturally control HIV and maintain good host immunity. Their mechanism is related to viruses, host genetic characteristics, immune response and other factors, but the relationship between the activation level of innate immunity and long-term non-progression is not clear. Therefore,we hypothesize that LTNPs may have some founction to control the HIV replication in vivo.In this study, we used RNA-seq to investigate the transcription（lncRNA, miRNA and mRNA) profiles of LTNPs and typical progressors (TPs).This study is showing expression spectrum of mRNA and non-coding RNA between HIV-infected long-term non-progressors and typical progressors, and analyze the role of mRNA,IncRNA and miRNA in the HIV infected long-term non-progression based on whole transcriptome level.This study reveals the mechanism of different disease progression between LTNPs and TPs through functional enrichment analysis and differentially expressed genes(DEGs) analysis.

2021-02-10 | E-MTAB-9997 | biostudies-arrayexpress

Proteomics Characterization of Longitudinal CSF from ALS Fast and Slow Progressors

Project description:Each CSF sample was concentrated and added to a multiple affinity removal spin cartridge .The eluates were subjected to a buffer exchange and proteins were reduced using DTT and alkylated using iodoacetamide. Digestions were performed using trypsin. CSF from patients with amyotrophic lateral sclerosis (ALS) was characterized via LC-MS/MS. Biomarkers were identified to differentiate fast and slow progressors.

2024-01-26 | PXD035026 | Pride

Genome-wide analysis of gene expression in whole blood from HIV-1 progressors and non-progressors.

Project description:Expression profiling of whole blood from HIV-1 progressors and non-progressors. The comparison results showed differently expressed genes associated with disease progress.

2014-09-06 | GSE56837 | GEO

Genome-wide analysis of gene expression in whole blood from HIV-1 progressors and non-progressors.

Project description:Expression profiling of whole blood from HIV-1 progressors and non-progressors. The comparison results showed differently expressed genes associated with disease progress. Total RNA obtained from whole blood from 26 patients differently numbered.

2014-09-06 | E-GEOD-56837 | biostudies-arrayexpress

Early PD-1 expression on CD8+ T-cells and the development of a stem-like phenotype are associated with HIV slow-progression in children

Project description:Population RNAseq was performed in PD-1high CD8+ T-cell of RP (rapid progressors) and SP (slow progressors) in the timepoint immediately after analytical treatment interruption. Although large proportion of differentially expressed genes appeared to be driven by individual heterogeneity, the RPs showed higher levels of Tim-3 (HAVCR2), a marker when co-expressed with PD-1+ that also characterizes terminally exhausted CD8+ T-cells

2023-02-01 | GSE221366 | GEO

Comparison of transcriptional profiles of PBMCs from HIV-infected rapid progressors and chronic progressors

Project description:A significant percentage of HIV-infected individuals experience a sharp decline in CD4+ T cell counts and progress to AIDS quickly after primary infection. Identification of biomarkers distinguishing rapid progressors (RPs) versus chronic progressors (CPs) is critical for early clinical intervention and could provide novel strategies to facilitate vaccine design and immune therapy. mRNA and miRNA expression profiles in the peripheral blood mononuclear cells (PBMCs) of RPs and CPs were investigated at 111±22 days (Mean±SD) of HIV infection. The association of mRNA and miRNA expression with disease progression was examined by receiver operating characteristic analysis and Kaplan-Meier survival analysis. Pathway enrichment analysis showed that genes with deregulated expression in RPs are primarily involved in apoptosis pathways. Furthermore, we found that 5 miRNAs (miR-31, -200c, -526a, -99a and -503) in RPs were significantly decreased compared to those in CPs (P<0.05). The decreased expression of these miRNAs was associated with rapid disease progression of HIV infection with a 94% predictive value as measured by the area under the curve. The upregulated predicted targets from the 5 signature miRNAs and all upregulated genes identified from mRNA microarray converged to the apoptosis pathway. Moreover, overexpression of miR-31 in primary human T cells promoted their survival. Our results have identified a distinct transcriptomic signature in PBMCs of RPs and provided novel insights to the pathogenesis of HIV infection.

2013-05-01 | GSE44216 | GEO

Comparison of transcriptional profiles of PBMCs from HIV-infected rapid progressors and chronic progressors

Project description:A significant percentage of HIV-infected individuals experience a sharp decline in CD4+ T cell counts and progress to AIDS quickly after primary infection. Identification of biomarkers distinguishing rapid progressors (RPs) versus chronic progressors (CPs) is critical for early clinical intervention and could provide novel strategies to facilitate vaccine design and immune therapy. mRNA and miRNA expression profiles in the peripheral blood mononuclear cells (PBMCs) of RPs and CPs were investigated at 111Â±22 days (MeanÂ±SD) of HIV infection. The association of mRNA and miRNA expression with disease progression was examined by receiver operating characteristic analysis and Kaplan-Meier survival analysis. Pathway enrichment analysis showed that genes with deregulated expression in RPs are primarily involved in apoptosis pathways. Furthermore, we found that 5 miRNAs (miR-31, -200c, -526a, -99a and -503) in RPs were significantly decreased compared to those in CPs (P<0.05). The decreased expression of these miRNAs was associated with rapid disease progression of HIV infection with a 94% predictive value as measured by the area under the curve. The upregulated predicted targets from the 5 signature miRNAs and all upregulated genes identified from mRNA microarray converged to the apoptosis pathway. Moreover, overexpression of miR-31 in primary human T cells promoted their survival. Our results have identified a distinct transcriptomic signature in PBMCs of RPs and provided novel insights to the pathogenesis of HIV infection. A cohort of primary HIV infected individuals with different disease outcome were enrolled in this study. We included 6 individuals with rapid disease progression (RP), seven with chronic disease progression (CP). The HIV infected individuals were never on therapy before the time of sample taken.

2013-05-01 | E-GEOD-44216 | biostudies-arrayexpress